ELISA Kit for Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- Product No.CEA482Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range6.17-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.71pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1).
No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Endothelin 1 (EDN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Endothelin 1 (EDN1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endothelin 1 (EDN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Heart and Circulatory Physiology||B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness APS: source|
|PLOS ONE||CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source|
|36||Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 PubMed: PMC3695508|
|Clinics (Sao Paulo).||Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice Pubmed: 24212843|
|Life Sci.||Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829|
|Transpl Int.||One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys Pubmed:24977654|
|J Endocrinol Invest.?||The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337|
|Chemical Research in Toxicology||Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880|
|Journal of Surgical Research||Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning Sciencedirect:S0022480414004909|
|Pharmaceutical Biology||The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil PubMed: 26023989|
|A Periodical of the Hungarian Academy of Sciences||The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients PubMed: 26690031|
|Chemical Research in Toxicology||Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880|
|Human & Experimental Toxicology||An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury PubMed: 26078283|
|Artery Research||Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome science:article|
|Transplant proc||Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients. pubmed:27496444|
|Biomed Journal||Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. pubmed:27884380|
|International Journal of Advanced Research in Biological Sciences||Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose pdfcopy:42552|
|Journal of Drug Metabolisn& Toxicology||Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose 587e5b6508aed3826af45e2d.pdf|
|Polish Journal of Veterinary Sciences||Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema pubmed:27760029|
|Artery Research||Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome article:S1872-9312(16)30085-0|
|Journal of Molecular Biomarkers J & Diagnosis||Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses DOI: 10.4172/2155-9929.1000318|
|A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats. AN=123819331|
|Journal of Functional Foods||Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation 10.1016/j.jff.2017.05.033|
|Radiation Research||Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute … Pubmed:29809108|
|Scandinavian Journal of Clinical and Laboratory Investigation||Isolated kidney perfusion: the influence of pulsatile flow Pubmed:29301417|
|Endokrynologia Polska||Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension … Pubmed: 30359461|
|Journal of Pharmacological Sciences||Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783|
|Molecular Nutrition & Food Research||Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight … Pubmed: 30570214|
|Journal of Cosmetic Dermatology||Whitening effects of cosmetic formulation in the vascular component of skin pigmentation Pubmed: 31074159|
|Eurasian Journal of Clinical Sciences||APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan|
|Journal of Functional Foods||Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease|
|Scientific Reports||Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735|
|Evidence-Based Complementary and Alternative Medicine||Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats Pubmed: 33133215|
|Catalog No.||Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species||Applications (RESEARCH USE ONLY!)|
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