ELISA Kit for Neuropeptide S (NPS)

NP-S

Specificity

This assay has high sensitivity and excellent specificity for detection of Neuropeptide S (NPS).
No significant cross-reactivity or interference between Neuropeptide S (NPS) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Neuropeptide S (NPS) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuropeptide S (NPS) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-102 90
EDTA plasma(n=5) 79-104 84
heparin plasma(n=5) 78-99 81

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide S (NPS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide S (NPS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuropeptide S (NPS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 85-101% 88-95% 92-101%
EDTA plasma(n=5) 90-102% 98-105% 94-102% 96-105%
heparin plasma(n=5) 93-101% 93-105% 92-99% 99-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Nordic Journal of Psychiatry Investigating the relationship between plasma neuropeptide-S levels and clinical depression Pubmed:29488437
Archives of Neuropsychiatry Plasma Neuropeptide-S Levels in Populations Diagnosed with Generalized Anxiety Disorder: A Controlled Study.
Neurological Sciences The effects of IVIg therapy on serum levels of neuropeptide Y and cytokines in Guillain-Barré syndrome Pubmed: 31494821
Respiratory Physiology & Neurobiology Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Pubmed: 31542455
Neuroscience Role of Neuropeptide S on behavioural and neurochemical changes of an animal model of attention-deficit/hyperactivity disorder Pubmed: 32976984
Sci Transl Med Neuropeptide S receptor 1 is a nonhormonal treatment target in endometriosis 34433639
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