ELISA Kit for Parathyroid Hormone (PTH)

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

Specificity

This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH).
No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Parathyroid Hormone (PTH) and the recovery rates were calculated by comparing the measured value to the expected amount of Parathyroid Hormone (PTH) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-9183
EDTA plasma(n=5)86-10297
heparin plasma(n=5)82-9485

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Parathyroid Hormone (PTH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)89-101%78-94%81-95%85-92%
EDTA plasma(n=5)82-98%81-93%79-98%97-105%
heparin plasma(n=5)95-103%95-102%79-97%89-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1Assay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
Reagent Diluent1×300µLStop Solution1×6mL
TMB Substrate1×9mLInstruction manual1
Wash Buffer (30 × concentrate)1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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MagazineReference
Biological trace element researchElevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient DietPubMed: 19915804
The Korean Journal of NutritionEffects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized RatsKoreaMed: source
Acta Physiologica HungaricaWhole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parametersPubmed: 22173025
Toxicology and Industrial HealthThe effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709
Indian Journal of Experimental Biology Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats.PubMed: 22439438
Plos oneIs Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia?PubMed: PMC3679169
Nutr Hosp. Effect of the “protein diet” and bone tissue.Pubmed: 24483972
Academic JournalEffect of whole body vibration on healthy rat plasma parametersEbscohost: Source
J Nutr Sci Vitaminol (Tokyo)The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized RatsPubMed: 26639847
The Korean Society of PharmacognosyEffects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized RatArticle: Hksobf_2015_V46N2_167
J Bone Miner ResFat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult RatsPubmed:26175082
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