ELISA Kit for Serum Amyloid A (SAA)
SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
- Product No.CEA885Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range625-10,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 251ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Serum Amyloid A (SAA).
No significant cross-reactivity or interference between Serum Amyloid A (SAA) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Serum Amyloid A (SAA) and the recovery rates were calculated by comparing the measured value to the expected amount of Serum Amyloid A (SAA) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Serum Amyloid A (SAA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Nitric Oxide.||Effect of feeding a high fat diet on hydrogen sulfide (H 2 S) metabolism in the mouse Pubmed:24637018|
|Open Biochem J.||Acute Phase Response: Implication in ST-segment Elevation Myocardial Infarction Pubmed:24894970|
|Iran J Pharm Res.||Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial Pubmed:25237354|
|US National Library of Medicine National Institutes of Health||No association between vitamin D levels and inflammation markers in patients with acute coronary syndrome Pubmed:25615956|
|BMC Complementary and Alternative Medicine||Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121|
|BOSCA||EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWS Tcrjournals:Source|
|Life Sciences||Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet PubMed: 26188592|
|Endocrine||Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitus PubMed: 26049370|
|J Assist Reprod Genet.||The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome PubMed: 26463878|
|International Journal of Clinical and Experimental Medicine||Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhage files:ijcem0021201|
|PLoS One.||An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down Syndrome Pubmed:27023444|
|United States Patent Application||PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME y2016:0139148.html|
|Veterinary World||Acute phase response in lame crossbred dairy cattle pubmed:27956769|
|Nature Communications||A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense Pubmed:29410422|
|Journal of Animal Sciences||Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations Pubmed:30060086|
|Journal of Animal Science||Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood … Pubmed: 30184125|
|Journal of Dairy Science||Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows Pubmed: 17430930|
|European Review for Medical and Pharmacological Sciences||The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. Pubmed: 31298341|
|Research in Veterinary Science||Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows|
|Journal of Animal Science||Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers Pubmed: 31410465|
|PLoS One||Effects of vaccination on acute-phase protein response in broiler chicken Pubmed: 32191767|
|VETERINARY PATHOLOGY||AA Amyloid Deposition in the Central and Peripheral Nervous Systems in Flamingos Pubmed: 32677873|
|Comp Immunol Microbiol Infect Dis||Serum amyloid A as an marker of cow֨ s mastitis caused by Streptococcus sp. Pubmed: 32505957|
|Indian Journal of Animal Sciences||Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …|
|РОЛЬ СЫВОРОТОЧНОГО АМИЛОИДА А И С-РЕАКТИВНОГО БЕЛКА В ПРОГНОЗИРОВАНИИ ПОСЛЕОПЕРАЦИОННЫХ ОСЛОЖНЕНИЙ И …|
|JOURNAL OF ANIMAL SCIENCE||Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡ 33474564|
|Animals||Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid 34070499|
|peerj||Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats 33510972|
|Arquivo Brasileiro de Medicina Veterin¨¢ria e Zootecnia||CCS e amiloide A do leite de quartos mam¨¢rios tratados na secagem com plasma rico em plaquetas aut¨®logo, associado ou n?o a antibi¨®tico|
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