ELISA Kit for Gastrin (GT)

GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III


This assay has high sensitivity and excellent specificity for detection of Gastrin (GT).
No significant cross-reactivity or interference between Gastrin (GT) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Gastrin (GT) and the recovery rates were calculated by comparing the measured value to the expected amount of Gastrin (GT) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-102 93
EDTA plasma(n=5) 89-104 96
heparin plasma(n=5) 98-105 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastrin (GT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastrin (GT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gastrin (GT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 99-105% 82-91% 92-99%
EDTA plasma(n=5) 89-97% 88-95% 79-101% 99-105%
heparin plasma(n=5) 80-98% 98-105% 92-101% 96-105%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Journal of Diabetes Omeprazole improves the antiobesity and antidiabetic activity of Exendin-4 in db/db mice. PubMed: 22830490
Cell reports Liver-Derived Systemic Factors Drive b Cell Hyperplasia in Insulin-Resistant States PubMed: PMC3655439
Journal of Pharmacy and Pharmacology Lansoprazole enhances the antidiabetic effect of sitagliptin in mice with diet-induced obesity and healthy human subjects Pubmed: 24628303
Pharmacological Reports Combination of omeprazole with GLP-1 agonist therapy improves insulin sensitivity and antioxidant activity in liver in type 1 diabetic mice Pubmed: 24145087
World J Gastroenterol Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide NCBI: PMC4000510
世界华人消化杂志 清醒状态下热射病大鼠胃肠动力与胃泌素、胃动素的变化 Wjgnet:Source
World J Gastroenterol Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732
Nanoscale Engineering bioinspired bacteria-adhesive clay nanoparticles with a membrane-disruptiveproperty for the treatment of Helicobacter pylori infection. pubmed:27605059
Nutrients The Effect of Salt Intake and Potassium Supplementation on Serum Gastrin Levels in Chinese Adults: A Randomized Trial pubmed:28420122
Applied Biological Chemistry Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3
Science of Food and Agriculture Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer Pubmed: 30479041
Journal of tranditional chinese medical sciences Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB224Hu01 Recombinant Gastrin (GT) Positive Control; Immunogen; SDS-PAGE; WB.
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