ELISA Kit for Hemoglobin Delta (HBd)
- Product No.CED092Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, erythrocyte lysates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range2.47-200ug/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.95ug/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 700
For more details, please contact local distributors! US$ 3150
For more details, please contact local distributors! US$ 5950
For more details, please contact local distributors! US$ 49000
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Hemoglobin Delta (HBd).
No significant cross-reactivity or interference between Hemoglobin Delta (HBd) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Hemoglobin Delta (HBd) and the recovery rates were calculated by comparing the measured value to the expected amount of Hemoglobin Delta (HBd) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin Delta (HBd) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin Delta (HBd) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hemoglobin Delta (HBd) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY||Proteomic Analysis of Amniotic Fluid Proteins for Predicting the Outcome of Emergency Cerclage in Women with Cervical Insufficiency Pubmed: 30076666|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPD092Hu01||Recombinant Hemoglobin Delta (HBd)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAD092Hu01||Polyclonal Antibody to Hemoglobin Delta (HBd)||WB; IHC; ICC; IP.|
|CED092Hu||ELISA Kit for Hemoglobin Delta (HBd)||Enzyme-linked immunosorbent assay for Antigen Detection.|