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Human SH-SY5Y cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human NES Monoclonal Antibody (Catalog FAA500Hu06, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

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Anti-Nestin (NES) Monoclonal Antibody

Product No.FAA500Hu06
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
Potency (Clone Number)D5
Ig Isotype IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Excitation Laser
Vol. per test
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Organism Species Moren/a
ApplicationsFlow cytometry.
Downloadn/a
UOM 25test50test 100test 200test 500test
FOB US$ US$ US$ US$ US$
For more details, please contact local distributors!

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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Magazine	Citations
Kidney and Blood Pressure Research	Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5 Pubmed:29689548
Neural Plast	P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury Pubmed:35154311

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA500Hu01	Recombinant Nestin (NES)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA500Hu01	Polyclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
LAA500Hu51	APC-Linked Polyclonal Antibody to Nestin (NES)	WB; IHC; ICC; IF.
MAA500Hu27	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu21	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu23	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu24	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu25	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu22	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
FAA500Hu02	Anti-Nestin (NES) Monoclonal Antibody	FCM
FAA500Hu08	Anti-Nestin (NES) Monoclonal Antibody	Flow cytometry.
FAA500Hu04	Anti-Nestin (NES) Monoclonal Antibody	Flow cytometry.
FAA500Hu06	Anti-Nestin (NES) Monoclonal Antibody	Flow cytometry.
SEA500Hu	ELISA Kit for Nestin (NES)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA500Hu	Multiplex Assay Kit for Nestin (NES) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.