High Sensitive ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
- Product No.HEA798Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.9pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 756
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For more details, please contact local distributors! US$ 52920
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Hypoxia Inducible Factor 1 Alpha (HIF1a).
No significant cross-reactivity or interference between High Sensitive Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Hypoxia Inducible Factor 1 Alpha (HIF1a) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Hypoxia Inducible Factor 1 Alpha (HIF1a) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Hypoxia Inducible Factor 1 Alpha (HIF1a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Cancer Chemotherapy and Pharmacology||Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. PubMed: 19466611|
|European Journal of Pharmacology||Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition ScienceDirect: S0014299909011352|
|PLoS ONE||Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis PubMed: PMC3781153|
|International Journal of Molecular Medicine||Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats Pubmed: 24002353|
|Canadian Journal of Physiology and Pharmacology||Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia Pubmed: 24289078|
|US8569372 B2||Method for preventing or treating a disease, disorder or condition induced by retina ischemia US8569372|
|J Surg Res.||Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. Pubmed: 23816242|
|PLoS One.||HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source|
|Journal of Physiological Anthropology||Telomeres are elongated in rats exposed to moderate altitude Pubmed:Pmc4088304|
|Molecular Medicine Reports||Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. Pubmed:25384583|
|International Journal of Pharmaceutics||The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF Pubmed:25445537|
|International Journal of Molecular Medicine||Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats Pubmed:25524396|
|International Journal of Developmental Neuroscience||Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth PubMed: 25889224|
|Eur Surg Res||Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys PubMed: 25341590|
|Sci Rep||Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system PubMed: 25905434|
|Evidence-Based Complementary and Alternative Medicine||Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae pmc:PMC4794592|
|Biol Trace Elem Res.||Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α Pubmed:27121973|
|Раны и раневые инфекции.||Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией 91|
|Biological Trace Element Research||Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α. pubmed:27121973|
|Experimental and Therapeutic Medicine||Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis etm:13|
|Clinical Trials in Degenerative Diseases||Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial issn:2542-3975|
|Annals of Clinical & Laboratory Science||R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme pubmed:28667042|
|Archives of Gynecology and Obstetrics||Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? 10.1007/s00404-017-4461-2|
|EXPERIMENTAL AND THERAPEUTIC MEDICINE||Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee pubmed:28123469|
|Medical Journal Armed Forces India||Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation 10.1016/j.mjafi.2017.04.003|
|Journal of Physiological Anthropology||Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia Pubmed:29454386|
|Bulletin of Experimental Biology and Medicine||Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia Pubmed:29308565|
|Microchimica Acta||Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticles Pubmed: 30209641|
|Bulletin of Experimental Biology and Medicine||Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … Pubmed: 30353332|
|Translational Oncology||OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor Growth Pubmed: 30468988|
|West Indian Medical Journal||Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.|
|Bulletin of Experimental Biology and Medicine||Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats Pubmed: 30903510|
|The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency|
|scandinavian journal of immunology||Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathway Pubmed: 31055848|
|JOURNAL OF PERIODONTOLOGY||A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A … Pubmed: 31020669|
|Tissue Eng Part A||Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation Pubmed: 31238789|
|ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ|
|molecular and cellular biochemistry||Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia Pubmed: 31420792|
|Molecules||Regulation of MicroRNA-155 and Its Related Genes Expression by Inositol Hexaphosphate in Colon Cancer Cells Pubmed: 31744065|
|Stem Cell Res Ther||Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus Pubmed: 32894200|
|J Cell Physiol||PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancer Pubmed: 32383180|
|Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза|