High Sensitive ELISA Kit for Alpha-2-Macroglobulin (a2M)

A2MG; A2mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Alpha-2-Macroglobulin (a2M).
No significant cross-reactivity or interference between High Sensitive Alpha-2-Macroglobulin (a2M) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant High Sensitive Alpha-2-Macroglobulin (a2M) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Alpha-2-Macroglobulin (a2M) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 94-102 98
heparin plasma(n=5) 78-96 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Alpha-2-Macroglobulin (a2M) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-102% 83-101% 95-103% 94-101%
EDTA plasma(n=5) 96-103% 96-105% 90-105% 96-105%
heparin plasma(n=5) 78-89% 90-99% 99-105% 93-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular &amp; Cellular proteomics Semi-quantitative measurement of a specific glycoform using a DNA-tagged antibody and lectin affinity chromatography for glyco-biomarker development Pubmed:25525205
Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome PubMed: 26351173
Biological Trace Element Research Comparative Serum Proteomic Analysis of the Effects of Sodium Selenate on a Mouse Model of Alzheimer's Disease Pubmed: 30790121
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB017Hu01 Recombinant Alpha-2-Macroglobulin (a2M) Positive Control; Immunogen; SDS-PAGE; WB.
PAB017Hu01 Polyclonal Antibody to Alpha-2-Macroglobulin (a2M) WB; IHC; ICC; IP.
LAB017Hu71 Biotin-Linked Polyclonal Antibody to Alpha-2-Macroglobulin (a2M) WB; IHC; ICC.
MAB017Hu21 Monoclonal Antibody to Alpha-2-Macroglobulin (a2M) WB; IHC; ICC; IP.
MAB017Hu22 Monoclonal Antibody to Alpha-2-Macroglobulin (a2M) WB; IHC; ICC; IP.
HEB017Hu High Sensitive ELISA Kit for Alpha-2-Macroglobulin (a2M) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB017Hu ELISA Kit for Alpha-2-Macroglobulin (a2M) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB017Hu CLIA Kit for Alpha-2-Macroglobulin (a2M) Chemiluminescent immunoassay for Antigen Detection.
PSB017Hu01 Antibody Pair for Alpha-2-Macroglobulin (a2M) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB017Hu01 ELISA Kit DIY Materials for Alpha-2-Macroglobulin (a2M) Main materials for "Do It (ELISA Kit) Yourself".