High Sensitive ELISA Kit for Tau Protein (MAPT)
DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; Neurofibrillary tangle protein; Microtubule Associated Protein Tau; Paired helical filament-tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
- Product No.HEB983Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.8pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of High Sensitive Tau Protein (MAPT).
No significant cross-reactivity or interference between High Sensitive Tau Protein (MAPT) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Tau Protein (MAPT) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Tau Protein (MAPT) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Tau Protein (MAPT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Tau Protein (MAPT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Tau Protein (MAPT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Eur Rev Med Pharmacol Sci.||Effect of etanercept and lithium chloride on preventing secondary tissue damage in rats with experimental diffuse severe brain injury Pubmed:24452937|
|Pediatria i Medycyna Rodzinna||Wpływ omdlenia u młodocianych na stężenie białka tau w surowicy krwi b09f7df8-1e7d-4665-a2a5-bf54dae0cfe9|
|Acta Angiologica||Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy AA.2016.0008|
|Journal of physiology and pharmacology||THE PRESENCE OF TAU PROTEIN IN BLOOD AS A POTENTIAL PROGNOSTIC FACTOR IN STROKE PATIENTS pubmed:28011949|
|Acta Angiol||Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy AA.2016.0008|
|Frontiers in Aging Neuroscience||Differential hyperphosphorylation of tau-S199,-T231 and-S396 in organotypic brain slices of Alzheimer mice. A model to study early tau hyperphosphorylation using … Pubmed:29725295|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB983Hu01||Recombinant Tau Protein (MAPT)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB983Hu01||Polyclonal Antibody to Tau Protein (MAPT)||WB; IHC; ICC; IP.|
|MAB983Hu22||Monoclonal Antibody to Tau Protein (MAPT)||WB; IHC; ICC; IP.|
|SEB983Hu||ELISA Kit for Tau Protein (MAPT)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEB983Hu||Mini Samples ELISA Kit for Tau Protein (MAPT)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEB983Hu||High Sensitive ELISA Kit for Tau Protein (MAPT)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB983Hu01||ELISA Kit DIY Materials for Tau Protein (MAPT)||Main materials for "Do It (ELISA Kit) Yourself".|