High Sensitive ELISA Kit for Transferrin (TF)
TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
- Product No.HEC036Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.069ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 469
For more details, please contact local distributors! US$ 2111
For more details, please contact local distributors! US$ 3987
For more details, please contact local distributors! US$ 32830
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Transferrin (TF).
No significant cross-reactivity or interference between High Sensitive Transferrin (TF) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Transferrin (TF) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Transferrin (TF) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Transferrin (TF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Transferrin (TF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Transferrin (TF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|PLoS ONE||Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance PubMed: PMC3754973|
|Physiological research||The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease pubmed:27782743|
|Journal of Proteomics||Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921|
|Journal of Clinical Medicine||Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327|
|The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria||Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria|
|Haematologica||New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia Pubmed: 30792208|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|NPC036Hu01||Native Transferrin (TF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPC036Hu01||Recombinant Transferrin (TF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC036Hu05||Polyclonal Antibody to Transferrin (TF)||WB; IHC; ICC; IP.|
|PAC036Hu01||Polyclonal Antibody to Transferrin (TF)||WB; IHC; ICC; IP.|
|LAC036Hu81||FITC-Linked Polyclonal Antibody to Transferrin (TF)||WB; IHC; ICC; IF.|
|LAC036Hu71||Biotin-Linked Polyclonal Antibody to Transferrin (TF)||WB; IHC; ICC.|
|MAC036Hu21||Monoclonal Antibody to Transferrin (TF)||WB; IHC; ICC; IP.|
|MAC036Hu22||Monoclonal Antibody to Transferrin (TF)||WB; IHC; ICC; IP.|
|LAC036Hu72||Biotin-Linked Monoclonal Antibody to Transferrin (TF)||WB; IHC; ICC.|
|LAC036Hu82||FITC-Linked Monoclonal Antibody to Transferrin (TF)||WB; IHC; ICC; IF.|
|SEC036Hu||ELISA Kit for Transferrin (TF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEC036Hu||High Sensitive ELISA Kit for Transferrin (TF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC036Hu||CLIA Kit for Transferrin (TF)||Chemiluminescent immunoassay for Antigen Detection.|
|KSC036Hu01||ELISA Kit DIY Materials for Transferrin (TF)||Main materials for "Do It (ELISA Kit) Yourself".|