Instant ELISA Kit for Integrin Beta 1 (ITGb1)
CD29; MSK12; ITG-B1; FNRB; GPIIA; MDF2; MSK12; VLAB; Fibronectin Receptor Beta Polypeptide; Antigen CD29 Includes MDF2; Glycoprotein IIa; VLA-4 subunit beta
- Product No.IEB042Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length1h, 10min
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.31ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Instant Integrin Beta 1 (ITGb1).
No significant cross-reactivity or interference between Instant Integrin Beta 1 (ITGb1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Integrin Beta 1 (ITGb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Integrin Beta 1 (ITGb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Clinica Chimica Acta||Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955|
|Cancer Cell International||Epidermal growth factor-mediated Rab25 pathway regulates integrin β1 trafficking in colon cancer Pubmed:29515334|
|Cellular Signalling||Tubulin-dependent secretion of S100A6 and cellular signaling pathways activated by S100A6-integrin β1 interaction Pubmed:29020611|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB042Hu02||Recombinant Integrin Beta 1 (ITGb1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB042Hu01||Recombinant Integrin Beta 1 (ITGb1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB042Hu01||Polyclonal Antibody to Integrin Beta 1 (ITGb1)||WB; IHC; ICC; IP.|
|PAB042Hu02||Polyclonal Antibody to Integrin Beta 1 (ITGb1)||WB; IHC; ICC; IP.|
|LAB042Hu71||Biotin-Linked Polyclonal Antibody to Integrin Beta 1 (ITGb1)||WB; IHC; ICC.|
|MAB042Hu22||Monoclonal Antibody to Integrin Beta 1 (ITGb1)||WB; IHC; ICC; IP.|
|SEB042Hu||ELISA Kit for Integrin Beta 1 (ITGb1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|IEB042Hu||Instant ELISA Kit for Integrin Beta 1 (ITGb1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB042Hu01||ELISA Kit DIY Materials for Integrin Beta 1 (ITGb1)||Main materials for "Do It (ELISA Kit) Yourself".|