ELISA Kit DIY Materials for Cyclic Adenosine Monophosphate (cAMP)

c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Cyclic Adenosine Monophosphate (cAMP). No significant cross-reactivity or interference between Cyclic Adenosine Monophosphate (cAMP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Int J Clin Exp Pathol Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway PubMed: 26617802
Experimental Dermatology Light Emitting Diodes Down‐reCavia (Guinea pig )lates Cathelicidin, Kallikrein, and Toll‐like Receptor 2 Expressions in Keratinocytes and Rosacea‐like Mouse Skin Pubmed:27315464
arteriosclerosis thrombosis and vascular biology P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis. pubmed:28062501
Taylor & Francis Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation 09540105.2017.1306494
international journal of biological macromolecules Immunomodulatory effect of APS and PSP is mediated by Ca2+-cAMP and TLR4/NF-κB signaling pathway in macrophage. pubmed:27732877
Front Pharmacol.  Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. pubmed:28261091
Allergologia et immunopathologia The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin pubmed:27955890
Journal of Enzyme Inhibition and Medicinal Chemistry Structural re-positioning, molecular modelling, oxidative degradation, and biological screening of linagliptin as adenosine 3 receptor (ADORA3) modulators … Pubmed:29768061
Frontiers in Physiology Astragaloside IV Inhibits Adipose Lipolysis and Reduces Hepatic Glucose Production via Akt Dependent PDE3B Expression in HFD-Fed Mice Pubmed:29410630
Nanoscience and Nanotechnology Letters Dobutamine Up-Regulates Aquaporin 5 Expression in Septic Pulmonary Edema Model In Vitro via cAMP-PKA/CREB Signaling Pathway 10.1166:nnl.2018.2602
PLoS One Tuberculous meningitis is associated with higher cerebrospinal HIV-1 viral loads compared to other HIV-1-associated meningitides Pubmed:29394269
Theriogenology Developmental competence of buffalo (Bubalus bubalis) denuded oocytes cocultured with cumulus cells: Protective role of cumulus cells Pubmed:30092373
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA003Ge11 BSA Conjugated Cyclic Adenosine Monophosphate (cAMP) Immunogen; SDS-PAGE; WB.
CPA003Ge21 OVA Conjugated Cyclic Adenosine Monophosphate (cAMP) Immunogen; SDS-PAGE; WB.
CPA003Ge41 HSA Conjugated Cyclic Adenosine Monophosphate (cAMP) Immunogen; SDS-PAGE; WB.
PAA003Ge01 Polyclonal Antibody to Cyclic Adenosine Monophosphate (cAMP) WB; IHC; ICC; IP.
LAA003Ge71 Biotin-Linked Polyclonal Antibody to Cyclic Adenosine Monophosphate (cAMP) WB; IHC; ICC.
LAA003Ge81 FITC-Linked Polyclonal Antibody to Cyclic Adenosine Monophosphate (cAMP) WB; IHC; ICC; IF.
MAA003Ge01 Monoclonal Antibody to Cyclic Adenosine Monophosphate (cAMP) WB; IHC; ICC; IP.
CEA003Ge ELISA Kit for Cyclic Adenosine Monophosphate (cAMP) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA003Ge11 ELISA Kit DIY Materials for Cyclic Adenosine Monophosphate (cAMP) Main materials for "Do It (ELISA Kit) Yourself".