ELISA Kit DIY Materials for Paraoxonase 1 (PON1)

ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1

  • Product No.KSA243Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.25-80ng/mL
  • Applicable Sensitivity0.51ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Paraoxonase 1 (PON1). No significant cross-reactivity or interference between Paraoxonase 1 (PON1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Digestive Diseases and Sciences Fatty Liver Is Associated with Recurrent Bacterial Infections Independent of Metabolic Syndrome PubMed: 20205513
Clinica Chimica Acta Persistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndrome PubMed: 21704607
Journal of Laparoendoscopic & Advanced Surgical Techniques Combined Serum Proteomic and Metabonomic Profiling After Laparoscopic Sleeve Gastrectomy in Children and Adolescents Wiley: source
PLoS One. High density lipoprotein structural changes and drug response in lipidomic profiles following the long-term fenofibrate therapy in the FIELD substudy PubMed: 21887280
Atherosclerosis Macrophage cholesterol efflux to plasma and HDL in subjects with low and high homocysteine levels: a FIELD substudy PubMed: 21696738
Lipids in Health and Disease Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome PubMed: PMC3480889
British Journal of Nutrition Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activities Pubmed: 23510814
Diabetes & Metabolic Syndrome Paraoxonase 1: A better atherosclerotic risk predictor than HDL in type 2 diabetes mellitus Pubmed: 23680251
Redox Report Paraoxonase responses to exercise and niacin therapy in men with metabolic syndrome Pubmed:25180827
Toxicol Ind Health. Influence of α-tocopherol and α-lipoic acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of rats Pubmed:25548375
RETOS. Nuevas Tendencias en Educación Física, Deporte y Recreación A single session of aerobic exercise influences paraoxonase 1 activity and concentration Articulo: 345738764042
PLoS One Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis PubMed: 25789864
Renal Failure Association of lipid profile with serum PON1 concentration in patients with chronic kidney disease Pubmed:26888625
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article
Clinical and Experimental Hypertension Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension pubmed:27668323
Diabetes & Metabolic Syndrome: Clinical Research & Reviews Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian population pubmed:29254890
Diabetes/Metabolism Research and Reviews A Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes Mellitus pubmed:29156090
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858
Pharmacological Reports The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707
American Journal of Food Science and Health Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats ISSN: 2381-7224
Metabolic brain disease Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels pubmed:28808839
Therapeutic Apheresis And Dialysis Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease Pubmed:29368426
Biological Trace Element Research Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats Pubmed: 30251228
GLYCOCONJUGATE JOURNAL Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer Pubmed: 30607521
Hematology. Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails Pubmed: 30661467
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy Pubmed: 31162828
Environment International Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures Pubmed: 31639605
Nutrition, Metabolism and Cardiovascular Diseases Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake Pubmed: 31757567
Environment International Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins Pubmed: 31783242
RESPIRATORY RESEARCH The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation … Pubmed: 33148285
CLINICAL RHEUMATOLOGY PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients Pubmed: 32556934
respiratory research The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555
PLoS One Vitamin D status and systemic redox biomarkers in adults with obesity 30973911
Oxid Med Cell Longev Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men 34917230
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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EPA243Mu61 Eukaryotic Paraoxonase 1 (PON1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA243Mu01 Polyclonal Antibody to Paraoxonase 1 (PON1) WB; IHC; ICC; IP.
PAA243Mu02 Polyclonal Antibody to Paraoxonase 1 (PON1) WB; IHC; ICC; IP.
LAA243Mu71 Biotin-Linked Polyclonal Antibody to Paraoxonase 1 (PON1) WB; IHC; ICC.
SEA243Mu ELISA Kit for Paraoxonase 1 (PON1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA243Mu Multiplex Assay Kit for Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA243Mu01 ELISA Kit DIY Materials for Paraoxonase 1 (PON1) Main materials for "Do It (ELISA Kit) Yourself".