ELISA Kit DIY Materials for Vasoactive Intestinal Peptide (VIP)

PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27


The Abs in the kit have high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP). No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Clinical Genetics VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population Wiley: source
Human immunology Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy Pubmed: 23800435
Headache: The Journal of Head and Face Pain CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine Onlinelibrary: head.12372
World J Gastroenterol Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide NCBI: PMC4000510
Cephalalgia? Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine Pubmed:24847167
J Huazhong Univ Sci Technolog Med Sci. Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients Pubmed:25318892
PLOS ONE Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters Pubmed:25647228
PLOS ONE Aqueous Fraction of PubMed: 25647228
Pharmacol Res Oral curcumin has anti-arthritic efficacy through somatostatin generation PubMed: 25836921
World J Gastroenterol Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732
Annals of Allergy, Asthma and Immunology Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis Pubmed:27156748
Cephalalgia. Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients Pubmed:27250233
Headache No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine pubmed:27634731
Sci Rep. Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. pubmed:28054652
BMC Complementary and Alternative Medicine [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice 10.1186/s12906-017-1903-0
Applied Biological Chemistry Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis Pubmed:28914594
Evidence-Based Complementary and Alternative Medicine Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids Pubmed:29853946
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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