ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMG1)

HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine High mobility group box protein-1 (HMGB-1) as a new diagnostic marker in patients with acute appendicitis PubMed: PMC3094252
Respiration Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice Karger: 000319151
Surgery Clinical relevance of single nucleotide polymorphisms of the high mobility group box 1 protein gene in patients with major trauma in Southwest China Pubmed: 22047946
Viral Immunology Is HMGB1 a New Indirect Marker for Revealing Fibrosis in Chronic Hepatitis and a New Therapeutic Target in Treatment? Wiley: source
Chinese Medical Journal Severity of sepsis is correlated with the elevation of serum high-mobility group box 1 in rats CMJ: 2009218808935901080
Microbiology and Immunology Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosis PubMed: 23278480
ACTA biochimica polonica High mobility group B1 levels in sepsis and disseminated intravascular coagulation Actabp: Source
Anesthesia and Analgesia The Inhibitory Effect of Lidocaine on the Release of High Mobility Group Box 1 in Lipopolysaccharide-Stimulated Macrophages PubMed: 21288974
Rheumatology International Elevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity and quality of life PubMed: 23143556
Inflammation Research Mammalian target of rapamycin complex 2 regulates inflammatory response to stress PubMed: 22899279
Acta Biochimica et Biophysica Sinica IGF-1 alleviates ox-LDL-induced inflammation via reducing HMGB1 release in HAECs PubMed: 22782142
LabVest The suppression of TRIM21 and the accumulation of IFN-α play crucial roles in the pathogenesis of osteonecrosis of the femoral head. PubMed: 22825687
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Evidence-based Complementary and Alternative Medicine ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 Expression PubMed: PMC3770047
Exp Ther Med Correlation between serum high-mobility group box-1 levels and high-sensitivity C-reactive protein and troponin I in patients with coronary artery disease Pubmed: 23935732
International of Rheumatic disesases Serum levels of high mobility group box 1 protein and its association with quality of life and psychological and functional status in patients with fibromyalgia Pubmed: 23901875
Surgery A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) Pubmed: 24468034
Acta Pharmacol Sin. Simvastatin suppresses vascular inflammation and atherosclerosis in ApoE-/- mice by downregulating the HMGB1-RAGE axis Pubmed: 23564080
BMC pulmonary medicine Soluble receptor for advanced glycation end-products and progression of airway disease Pubmed: 24758342
Biochemical Pharmacology Ascorbic acid reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and improves survival rate in septic mice by activation of Nrf2/HO-1 signals Pubmed:25896849
BioMed Research International Effects of Single or Combined Treatments with Radiation and Chemotherapy on Survival and Danger Signals Expression in Glioblastoma Cell Lines Hindawi:Source
J Cardiothorac Surg. High plasma levels of high mobility group box 1 is associated with the risk of sepsis in severe blunt chest trauma patients: a prospective cohort study Pubmed:Pmc4132233
J Leukoc Biol. HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells Pubmed:24374967
PLOS ONE The Alarmin Concept Applied to Human Renal Transplantation: Evidence for a Differential Implication of HMGB1 and IL-33 Pubmed:24586382
Journal of Surgical Research Plasma levels of high mobility group box 1 increase in patients with posttraumatic stress disorder after severe blunt chest trauma: a prospective cohort study Pubmed:25016440
Virus Research High-mobility group box-1, promising serological biomarker for the distinction of human WNV disease severity Pubmed:25197038
Journal of Stroke and Cerebrovascular Diseases Increased VEGF and Decreased SDF-1α in Patients with Silent Brain Infarction Are Associated with Better Prognosis after First-Ever Acute Lacunar Stroke Pubmed:25601176
中华临床医师杂志 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 Journal: Fulltext
Oxidative Medicine and Cellular Longevity Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1 Journals: Omcl
Int Immunopharmacol Hesperidin ameliorates lipopolysaccharide-induced acute lung injury in mice by inhibiting HMGB1 release PubMed: 25724384
Journal of Immunotherapy Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106
Atherosclerosis Retinoic acid inhibits tissue factor and HMGB1 via modulation of AMPK activity in TNF-α activated endothelial cells and LPS-injected mice PubMed: 26116962
PLOS ONE Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in China PubMed: 26317615
Int Immunopharmacol Betulin attenuates lung and liver injuries in sepsis PubMed: 26644168
journal of cardiochoracic surgery High mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgery PubMed: 26109393
Int Immunopharmacol Glycyrrhizin reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and endotoxemic mice by p38/Nrf2-dependent induction of HO-1 PubMed: 25812767
World J Gastroenterol Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro PubMed: 26167076
Neurotoxicology Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats PubMed: 25845300
Journal of Neuroimmunology HMGB1 expression patterns during the progression of experimental autoimmune encephalomyelitis PubMed: 25773152
J Neuroinflammation HMGB1 release triggered by the interaction of live retinal cells and uveitogenic T cells is Fas/FasL activation-dependent PubMed: 26394985
PLoS One Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-1 Pathway to Induce Inflammation Response PubMed: 25793984
Neuroscience Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice PubMed: 26363151
Cancer Biomarkers Serum levels of HMGB1 have a diagnostic role in metastatic renal cell cancer Pubmed:27062570
International Immunopharmacology Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways Pubmed:27179304
SpringerPlus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway. Pubmed:27026909
Acta Biochim Biophys Sin (Shanghai). High mobility group box1 protein is involved in acute inflammation induced by Clostridium difficile toxin A. content:2016
United States Patent Application COMPOSITIONS AND METHODS FOR INHIBITING HMGB1 ACTIVATION OF MELANOCYTES y2016:0077082.html
United States Patent Application METHOD OF MAKING A SKIN CARE COMPOSITION y2016:0077080.html
Oxid Med Cell Longev. Oxidative Stress in Metabolic Disorders: Pathogenesis, Prevention, and Therapeutics Pubmed:26949450
Oncotarget Zoledronic acid-encapsulating self-assembling nanoparticles and doxorubicin: a combinatorial approach to overcome simultaneously chemoresistance and immunoresistance in breast tumors pubmed:26980746
clinical biochemistry Relevance of pre-analytical blood management on the emerging cardiovascular protein biomarkers TWEAK and HMGB1 and on miRNA serum and plasma profiling. pubmed:27847340
disease markers Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. pubmed:28348451
Biogerontology. Changes in pro-inflammatory markers and leucine concentrations in response to Nordic Walking training combined with vitamin D supplementation in elderly women. pubmed:28316011
Journal of Immunology Research Serum HMGB1 serves as a novel laboratory indicator reflecting disease activity and pubmed:27800496
Springerplus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway pubmed:27026909
Molecular Immunology HMGB1 blockade differentially impacts pulmonary inflammation and defense responses in poly (I: C)/LPS-exposed heart transplant mice pubmed:27387278
Oxidative Medicine and Cellular Longevity Reduced HMGB 1-Mediated Pathway and Oxidative Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in … pubmed:27833703
BioMed Research International  High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A pubmed:27579314
Cardiology The role of high mobility group box 1 protein in interleukin-18-induced myofibroblastic transition of valvular interstitial cells pubmed:27395056
Innate immunity HMGB1 exacerbates experimental mouse colitis by enhancing innate lymphoid cells 3 inflammatory responses via promoted IL-23 production pubmed:27670944
Urolithiasis. Urinary MCP-1、HMGB1 increased in calcium nephrolithiasis patients and the influence ofhypercalciuria on the production of the two cytokines. pubmed:27393275
Journal of Investigative Surgery Diagnostic Value of High-Mobility Group Box 1 (HMGB1) Protein in Acute and Perforated Appendicitis. pubmed:27922766
J Cell Biochem High Mobility Group Box 1 Mediates Interferon-r-Induced Phenotypic Modulation of Vascular Smooth Muscle Cells. pubmed:27579780
BMC Vet Res. Blood markers of fibrinolysis and endothelial activation in canine babesiosis. pubmed:28363279
Immunol Res. Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells. pubmed:28108937
Biomed Pharmacother. SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. pubmed:28122311
Sci Rep.  Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats. pubmed:28373667
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