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ELISA Kit DIY Materials for Sirtuin 2 (SIRT2)

SIR2L; SIR2-L; SIR2L2; Silent Information Regulator 2 Protein; NAD-Dependent Deacetylase Sirtuin-2; Silent Mating Type Information Regulation 2 Homolog

  • Product No.KSA430Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.66ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Sirtuin 2 (SIRT2). No significant cross-reactivity or interference between Sirtuin 2 (SIRT2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Brain Research Bulletin The effects of melatonin and curcumin on the expression of SIRT2, Bcl-2 and Bax in the hippocampus of adult rats Pubmed:29325994
Сибирский федеральный университет Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA430Ra01 Recombinant Sirtuin 2 (SIRT2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA430Ra01 Polyclonal Antibody to Sirtuin 2 (SIRT2) WB; IHC; ICC; IP.
LAA430Ra71 Biotin-Linked Polyclonal Antibody to Sirtuin 2 (SIRT2) WB; IHC; ICC.
SEA430Ra ELISA Kit for Sirtuin 2 (SIRT2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA430Ra Multiplex Assay Kit for Sirtuin 2 (SIRT2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA430Ra01 ELISA Kit DIY Materials for Sirtuin 2 (SIRT2) Main materials for "Do It (ELISA Kit) Yourself".