≡

ELISA Kit DIY Materials for Caspase 14 (CASP14)

Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14

  • Product No.KSA457Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.061ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Caspase 14 (CASP14). No significant cross-reactivity or interference between Caspase 14 (CASP14) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Dermatological Science Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis Pubmed:25315296
In Vitro Cell Dev Biol Anim Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytes PubMed: 26427706
Applied Biochemistry and Biotechnology Enhanced Induction of Apoptosis in HaCaT Cells by Luteolin Encapsulated in PEGylated Liposomes—Role of Caspase-3/Caspase-14 Pubmed: 30390204
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA457Mu01 Recombinant Caspase 14 (CASP14) Positive Control; Immunogen; SDS-PAGE; WB.
RPA457Mu02 Recombinant Caspase 14 (CASP14) Positive Control; Immunogen; SDS-PAGE; WB.
PAA457Mu02 Polyclonal Antibody to Caspase 14 (CASP14) WB; IHC; ICC; IP.
PAA457Mu01 Polyclonal Antibody to Caspase 14 (CASP14) WB; IHC; ICC; IP.
SEA457Mu ELISA Kit for Caspase 14 (CASP14) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA457Mu Multiplex Assay Kit for Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA457Mu01 ELISA Kit DIY Materials for Caspase 14 (CASP14) Main materials for "Do It (ELISA Kit) Yourself".