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ELISA Kit DIY Materials for Podocin (PDCN)

NPHS2; NPHS-2; Nephrosis 2; Idiopathic Steroid-Resistant

  • Product No.KSA938Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, urine, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.29ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Podocin (PDCN). No significant cross-reactivity or interference between Podocin (PDCN) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788
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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA938Hu01 Recombinant Podocin (PDCN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA938Hu02 Recombinant Podocin (PDCN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA938Hu01 Polyclonal Antibody to Podocin (PDCN) WB; IHC; ICC; IP.
LAA938Hu71 Biotin-Linked Polyclonal Antibody to Podocin (PDCN) WB; IHC; ICC.
LAA938Hu81 FITC-Linked Polyclonal Antibody to Podocin (PDCN) WB; IHC; ICC; IF.
MAA938Hu22 Monoclonal Antibody to Podocin (PDCN) WB
MAA938Hu23 Monoclonal Antibody to Podocin (PDCN) WB
MAA938Hu21 Monoclonal Antibody to Podocin (PDCN) WB; IHC; ICC; IP.
SEA938Hu ELISA Kit for Podocin (PDCN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA938Hu Multiplex Assay Kit for Podocin (PDCN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA938Hu01 ELISA Kit DIY Materials for Podocin (PDCN) Main materials for "Do It (ELISA Kit) Yourself".