ELISA Kit DIY Materials for Ghrelin (GHRL)
MTLRP; Growth Hormone-Releasing Peptide
- Product No.KSA991Hu11
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range370.4-30,000pg/mL
- Applicable Sensitivity
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
96T*20 96T*50 96T*100
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The Abs in the kit have high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Archives of Oral Biology||Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescents PubMed: 21075359|
|Department of Medical Biochemistry and Clinical Biochemistry||Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533|
|Journal of Physiology and Pharmacology||Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone PubMed: 23211309|
|Gene||The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats PubMed: 22842192|
|Hormone Research in Paediatrics||Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and Anorexia Pubmed: 23774845|
|Surgical Innovation||Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine model Pubmed:25294792|
|Obesity Surgery||Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine Model Pubmed:25511751|
|Obes Surg||Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model PubMed: 26202420|
|Surg Innov||Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine Model PubMed: 25294792|
|Nutrients.||Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity? Pubmed:27240398|
|World Journal of Gastroenterology||Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats. pubmed:27621579|
|Ginekologia Polska||Increased serum ghrelin in preeclampsia: is ghrelin a friend or a foe? pubmed:27321099|
|Gynecological Endocrinology||The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellitus pubmed:27791436|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA991Hu01||Recombinant Ghrelin (GHRL)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CPA991Hu11||BSA Conjugated Ghrelin (GHRL)||Immunogen; SDS-PAGE; WB.|
|CPA991Hu21||OVA Conjugated Ghrelin (GHRL)||Immunogen; SDS-PAGE; WB.|
|PAA991Hu08||Polyclonal Antibody to Ghrelin (GHRL)||WB; IHC; ICC; IP.|
|PAA991Hu01||Polyclonal Antibody to Ghrelin (GHRL)||WB; IHC; ICC; IP.|
|LAA991Hu71||Biotin-Linked Polyclonal Antibody to Ghrelin (GHRL)||WB; IHC; ICC.|
|CEA991Hu||ELISA Kit for Ghrelin (GHRL)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA991Hu11||ELISA Kit DIY Materials for Ghrelin (GHRL)||Main materials for "Do It (ELISA Kit) Yourself".|