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ELISA Kit DIY Materials for Glutamate Decarboxylase 2 (GAD2)

GAD65; Glutamate Decarboxylase 2, Acid; Pancreatic Islets And Brain,65kDa; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase 65 kDa isoform

  • Product No.KSB258Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.27ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Glutamate Decarboxylase 2, Acid (GAD2). No significant cross-reactivity or interference between Glutamate Decarboxylase 2, Acid (GAD2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
International Journal of Reproductive Medicine Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis 33575317
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB258Hu01 Recombinant Glutamate Decarboxylase 2 (GAD2) Positive Control; Immunogen; SDS-PAGE; WB.
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KSB258Hu01 ELISA Kit DIY Materials for Glutamate Decarboxylase 2 (GAD2) Main materials for "Do It (ELISA Kit) Yourself".