ELISA Kit DIY Materials for Carnosine (Car)

β-Alanyl-L-Histidine

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Carnosine (Car). No significant cross-reactivity or interference between Carnosine (Car) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Springerplus Dietary supplemental vitamin B6 increases carnosine and anserine concentrations in the heart of rats PubMed: 26101732
Mol Nutr Food Res Zinc supplementation influences genomic stability biomarkers, antioxidant activity, and zinc transporter genes in an elderly Australian population with low zinc status PubMed: 25755079
Pediatric Diabetes The effect of 12 weeks carnosine supplementation on renal functional integrity and oxidative stress in pediatric patients with diabetic nephropathy: a randomized placebo-controlled trial pubmed:28744992
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPB450Ge11 BSA Conjugated Carnosine (Car) Immunogen; SDS-PAGE; WB.
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KSB450Ge11 ELISA Kit DIY Materials for Carnosine (Car) Main materials for "Do It (ELISA Kit) Yourself".