ELISA Kit DIY Materials for Caspase 1 (CASP1)

ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

  • Product No.KSB592Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates , cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.118ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Caspase 1 (CASP1). No significant cross-reactivity or interference between Caspase 1 (CASP1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434
Fertil Steril Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” PubMed: 26474737
PLoS One Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response. pubmed:28704412
Journal of Virology NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination Pubmed:29386291
Journal of Translational Medicine Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? Pubmed:29665864
Reproductive Sciences A role for the inflammasome in spontaneous labor at term Pubmed:27852921
Journal of Virology CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSES Pubmed: 29386291
ACTA Otorhinolaryngologica Italica NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media Pubmed: 30676848
American Journal of Reproductive Immunology Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women Pubmed: 31148259
Taiwanese Journal of Obstetrics & Gynecology Serum Caspase-1 levels in women with polycystic ovary syndrome Pubmed: 32127139
Iranian Journal of Allergy, Asthma and Immunology Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES
Norwegian Journal of development of the International Science CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER
LIFE SCIENCES The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation 34560082
Biol Reprod The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice 34632484
J Mol Neurosci TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion Injury Pubmed:35041191
Viruses Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network Dispersion Pubmed:35062292
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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KSB592Hu01 ELISA Kit DIY Materials for Caspase 1 (CASP1) Main materials for "Do It (ELISA Kit) Yourself".