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ELISA Kit DIY Materials for Surfactant Protein B (SP-B)

SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein

  • Product No.KSB622Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, lung lavage fluid and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.054ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2166  US$ 3899  US$ 7581  US$ 12996 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Surfactant Associated Protein B (SPB). No significant cross-reactivity or interference between Surfactant Associated Protein B (SPB) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Respiratory Physiology & Neurobiology Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery PubMed: 21736957
American Journal of Rhinology & Allergy Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps PubMed: 23406594
19 Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study PubMed: 20650982
Plos one Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins PubMed: PMC3551896
Plos one The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS PubMed: PMC3787032
Cancer Cell. Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models PubMed: PMC3406925
Respir Physiol Neurobiol Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project Pubmed: 23619193
PLOS ONE Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure Pubmed:25514679
Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge Opus4:Source
Int J Cardiol Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study PubMed: 26310985
Plos One Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) Pubmed:27337142
PLoS One The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. pubmed:27656877
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. pubmed:28101052
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid PMC5209370
Respiratory Physiology & Neurobiology Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers pubmed:28467885
Cellular Physiology and Biochemistry Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production pubmed:28743125
American Journal of Physiology. Lung Cellular and Molecular Physiology Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. pubmed:29167126
Respiratory research Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Pubmed:29747649
Respiratory Physiology & Neurobiology  Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan Pubmed:29548887
Lung  Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation Pubmed:29445934
American Journal of Physiology Lung Cellular andMolecular Physiology Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells Pubmed: 30211653
Respiratory Research Culture of human alveolar epithelial type II cells by sprouting Pubmed: 30340591
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB622Ra01 Recombinant Surfactant Protein B (SP-B) Positive Control; Immunogen; SDS-PAGE; WB.
PAB622Ra01 Polyclonal Antibody to Surfactant Protein B (SP-B) WB; IHC; ICC; IP.
MAB622Ra21 Monoclonal Antibody to Surfactant Protein B (SP-B) WB; IHC; ICC; IP.
SEB622Ra ELISA Kit for Surfactant Protein B (SP-B) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB622Ra Multiplex Assay Kit for Surfactant Protein B (SP-B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB622Ra01 ELISA Kit DIY Materials for Surfactant Protein B (SP-B) Main materials for "Do It (ELISA Kit) Yourself".