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ELISA Kit DIY Materials for Neuregulin 4 (NRG4)

HRG4; Pro-NRG4; Pro-neuregulin-4, membrane-bound isoform

  • Product No.KSC174Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Neuregulin 4 (NRG4). No significant cross-reactivity or interference between Neuregulin 4 (NRG4) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Metabolism A case–control study: Association between serum neuregulin 4 level and non-alcoholic fatty liver disease PubMed: 26476959
Diabetes Research and Clinical Practice Comparison of serum NeureCavia (Guinea pig )lin 4 (Nrg4) levels in adults with newly diagnosed type 2 diabetes mellitus and controls without diabetes Pubmed:27329015
Scientific Reports Short-chain fatty acids and inulin, but not guar gum, prevent diet-induced obesity and insulin resistance through differential mechanisms in mice. pubmed:28733671
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC174Hu01 Recombinant Neuregulin 4 (NRG4) Positive Control; Immunogen; SDS-PAGE; WB.
PAC174Hu01 Polyclonal Antibody to Neuregulin 4 (NRG4) WB; IHC; ICC; IP.
MAC174Hu23 Monoclonal Antibody to Neuregulin 4 (NRG4) WB; IHC; ICC; IP.
MAC174Hu24 Monoclonal Antibody to Neuregulin 4 (NRG4) WB; IHC; ICC; IP.
MAC174Hu22 Monoclonal Antibody to Neuregulin 4 (NRG4) WB; IHC; ICC; IP.
MAC174Hu21 Monoclonal Antibody to Neuregulin 4 (NRG4) WB
SEC174Hu ELISA Kit for Neuregulin 4 (NRG4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC174Hu Multiplex Assay Kit for Neuregulin 4 (NRG4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC174Hu01 ELISA Kit DIY Materials for Neuregulin 4 (NRG4) Main materials for "Do It (ELISA Kit) Yourself".