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ELISA Kit DIY Materials for Afamin (AFM)

ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

  • Product No.KSC284Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range3.12-200ng/mL
  • Applicable Sensitivity1.48ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Afamin (AFM). No significant cross-reactivity or interference between Afamin (AFM) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Proteomics 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer ScienceDirect: S1874391912004964
Clin Chim Acta Afamin — A pleiotropic glycoprotein involved in various disease states PubMed: 25892677
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068
Biomarkers in Medicine Urine afamin and afamin–creatinine ratio as biomarkers for kidney injury Pubmed: 30433819
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC284Hu01 Recombinant Afamin (AFM) Positive Control; Immunogen; SDS-PAGE; WB.
RPC284Hu02 Recombinant Afamin (AFM) Positive Control; Immunogen; SDS-PAGE; WB.
PAC284Hu01 Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC; IP.
PAC284Hu02 Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC; IP.
LAC284Hu71 Biotin-Linked Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC.
MAC284Hu22 Monoclonal Antibody to Afamin (AFM) WB; IHC; ICC; IP.
SEC284Hu ELISA Kit for Afamin (AFM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC284Hu Multiplex Assay Kit for Afamin (AFM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC284Hu01 ELISA Kit DIY Materials for Afamin (AFM) Main materials for "Do It (ELISA Kit) Yourself".