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ELISA Kit DIY Materials for Mitochondrial Uncoupling Protein 2 (UCP2)

SLC25A8; UCPH; Solute carrier family 25 member 8 ;Uncoupling Protein 2, Mitochondrial

  • Product No.KSC586Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Uncoupling Protein 2, Mitochondrial (UCP2). No significant cross-reactivity or interference between Uncoupling Protein 2, Mitochondrial (UCP2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Stem Cells FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism Induction PubMed: 22865656
Food Research International A 4-week consumption of medium roast and dark roast coffees affects parameters of energy status in healthy subjects Sciencedirect:S0963996914002403
The Journal of Maternal-Fetal & Neonatal Medicine The effect of delivery type on uncoupling protein-2 levels PubMed: 26479203
Life Sciences Vitamin C and E chronic supplementation differentially affect hepatic insulin signaling in rats Pubmed:29291420
REPRODUCTIVE TOXICOLOGY Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosis Pubmed: 32911041
Tissue Cell Vitamin B12 ameliorate Tramadol-induced oxidative stress, endocrine imbalance, apoptosis and NO/iNOS/NF-κB expression in Sprague Dawley through regulatory … 34923198
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC586Ra01 Recombinant Mitochondrial Uncoupling Protein 2 (UCP2) Positive Control; Immunogen; SDS-PAGE; WB.
PAC586Ra01 Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) WB; IHC; ICC; IP.
LAC586Ra71 Biotin-Linked Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) WB; IHC; ICC.
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SEC586Ra ELISA Kit for Mitochondrial Uncoupling Protein 2 (UCP2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC586Ra Multiplex Assay Kit for Mitochondrial Uncoupling Protein 2 (UCP2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC586Ra01 ELISA Kit DIY Materials for Mitochondrial Uncoupling Protein 2 (UCP2) Main materials for "Do It(ELISA Kit) Yourself"