ELISA Kit DIY Materials for Glucagon Like Peptide 2 (GLP2)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Glucagon Like Peptide 2 (GLP2). No significant cross-reactivity or interference between Glucagon Like Peptide 2 (GLP2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
European Journal of Nutrition Improvement in glucose tolerance and insulin sensitivity by probiotic strains of Indian gut originin high-fat diet-fed C57BL/6J mice. pubmed:27757592
Scientific Reports Promising Biomarkers of Environmental Enteric Dysfunction: A Prospective Cohort study in Pakistani Children Pubmed:29445110
Journal of Lipid Research Novel GPR120 agonist TUG891 modulates fat taste perception and preference and activates tongue-brain-gut axis in mice Pubmed: 31806728
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