Multiplex Assay Kit for E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay)

CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-102 93
EDTA plasma(n=5) 82-101 90
heparin plasma(n=5) 82-90 86
sodium citrate plasma(n=5) 91-104 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 87-94% 78-96% 84-104%
EDTA plasma(n=5) 98-105% 97-104% 79-101% 89-101%
heparin plasma(n=5) 79-99% 86-94% 88-102% 97-104%
sodium citrate plasma(n=5) 91-101% 87-104% 96-105% 81-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Shock Systemic involvement of high-mobility group box 1 protein and therapeutic effect of anti-high-mobility group box 1 protein antibody in a rat model of crush injury. PubMed: 22392147
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
Journal of Neuroimmunology Evaluation of the effects of Eserine and JWH-133 on brain dysfunction associated with experimental endotoxemia PubMed: 25867462
International Journal of Food Sciences and Nutrition Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products PubMed: 26401576
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422
journal of thoracic and cardiovascular surgery Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers Pubmed: 32812629
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827
Frontiers in Pharmacology Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy Pubmed: 32508661
Microvasc Res Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency 34119534
Anim Sci J Effect of dipeptide on intestinal peptide transporter 1 gene expression: An evaluation using primary cultured chicken intestinal epithelial cells 34309968
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis Pubmed:35126715
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA029Mu02 Recombinant E-selectin Positive Control; Immunogen; SDS-PAGE; WB.
RPA029Mu01 Recombinant E-selectin Positive Control; Immunogen; SDS-PAGE; WB.
PAA029Mu02 Polyclonal Antibody to E-selectin WB; IHC; ICC; IP.
PAA029Mu01 Polyclonal Antibody to E-selectin WB; IHC; ICC; IP.
SEA029Mu ELISA Kit for E-selectin Enzyme-linked immunosorbent assay for Antigen Detection.
SCA029Mu CLIA Kit for E-selectin Chemiluminescent immunoassay for Antigen Detection.
LMA029Mu Multiplex Assay Kit for E-selectin ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA029Mu01 ELISA Kit DIY Materials for E-selectin Main materials for "Do It (ELISA Kit) Yourself".