• Star-Product
ELISA Kit for Selectin, Endothelium (SELE)

CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E

Specificity

This assay has high sensitivity and excellent specificity for detection of Selectin, Endothelium (SELE).
No significant cross-reactivity or interference between Selectin, Endothelium (SELE) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Selectin, Endothelium (SELE) and the recovery rates were calculated by comparing the measured value to the expected amount of Selectin, Endothelium (SELE) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-95 92
EDTA plasma(n=5) 90-99 93
heparin plasma(n=5) 79-102 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selectin, Endothelium (SELE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selectin, Endothelium (SELE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Selectin, Endothelium (SELE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-103% 88-95% 91-105% 86-97%
EDTA plasma(n=5) 87-101% 79-90% 80-103% 82-91%
heparin plasma(n=5) 99-105% 97-105% 78-89% 89-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Shock Systemic involvement of high-mobility group box 1 protein and therapeutic effect of anti-high-mobility group box 1 protein antibody in a rat model of crush injury. PubMed: 22392147
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
Journal of Neuroimmunology Evaluation of the effects of Eserine and JWH-133 on brain dysfunction associated with experimental endotoxemia PubMed: 25867462
International Journal of Food Sciences and Nutrition Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products PubMed: 26401576
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA029Mu02 Recombinant Selectin, Endothelium (SELE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA029Mu01 Recombinant Selectin, Endothelium (SELE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA029Mu02 Polyclonal Antibody to Selectin, Endothelium (SELE) WB; IHC; ICC; IP.
PAA029Mu01 Polyclonal Antibody to Selectin, Endothelium (SELE) WB; IHC; ICC; IP.
SEA029Mu ELISA Kit for Selectin, Endothelium (SELE) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA029Mu CLIA Kit for Selectin, Endothelium (SELE) Chemiluminescent immunoassay for Antigen Detection.
KSA029Mu01 ELISA Kit DIY Materials for Selectin, Endothelium (SELE) Main materials for "Do It (ELISA Kit) Yourself".