Multiplex Assay Kit for Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay)

NAGase; MEA5; NCOAT; Hexosaminidase C; N-Acetylhexosaminidase; Hyaluronidase; Meningioma-Expressed Antigen 5; Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-102 99
EDTA plasma(n=5) 85-95 90
heparin plasma(n=5) 78-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-96% 87-95% 78-97% 88-102%
EDTA plasma(n=5) 97-105% 93-101% 80-101% 88-104%
heparin plasma(n=5) 78-90% 91-99% 95-102% 78-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:MGEA5) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Plos one Metal-on-Metal Hip Prostheses and Systemic Health: A Cross-Sectional Association Study 8 Years after Implantation PubMed: PMC3677913
Renal Failure Evaluation of the Process of Recycling and Renal Parenchymal Injury after ESWL with Metabolites Excreted in the Urine Pubmed: 23413820
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105
PLoS One Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats PubMed: 25909514
Ecotoxicol Environ Saf Melamine and oxalate coexposure induces early kidney tubular injury through mitochondrial aberrations and oxidative stress 34507040
Antioxidants Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 1464 34573096
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA069Ra02 Recombinant Meningioma Expressed Antigen 5 (MGEA5) Positive Control; Immunogen; SDS-PAGE; WB.
RPA069Ra01 Recombinant Meningioma Expressed Antigen 5 (MGEA5) Positive Control; Immunogen; SDS-PAGE; WB.
PAA069Ra01 Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) WB; IHC; ICC; IP.
PAA069Ra02 Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) WB; IHC; ICC; IP.
LAA069Ra71 Biotin-Linked Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) WB; IHC; ICC.
SEA069Ra ELISA Kit for Meningioma Expressed Antigen 5 (MGEA5) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA069Ra CLIA Kit for Meningioma Expressed Antigen 5 (MGEA5) Chemiluminescent immunoassay for Antigen Detection.
LMA069Ra Multiplex Assay Kit for Meningioma Expressed Antigen 5 (MGEA5) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.