Multiplex Assay Kit for Mucin 1 (MUC1) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; CA153; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Mucin 1 (MUC1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Mucin 1 (MUC1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 1 (MUC1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 1 (MUC1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:MUC1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Annals of surgical oncology Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734
J Hum Lact Host Defense Proteins in Breast Milk and Neonatal Yeast Colonization PubMed: 26116637
Breast Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator PubMed: 26446295
PLoS One. The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital Fluids Pubmed:27182834
Int J Clin Exp Med The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai files:ijcem0020163.pdf
Talanta Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification. pubmed:28107977
Cellular Microbiology An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
Bio-protocol Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA 10.21769:BioProtoc.2879
Biosensors and Bioelectronics Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review
Journal of Electroanalytical Chemistry A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA413Mu02 Recombinant Mucin 1 (MUC1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA413Mu01 Recombinant Mucin 1 (MUC1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA413Mu03 Recombinant Mucin 1 (MUC1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA413Mu01 Polyclonal Antibody to Mucin 1 (MUC1) WB; IHC; ICC; IP.
PAA413Mu02 Polyclonal Antibody to Mucin 1 (MUC1) WB; IHC; ICC; IP.
PAA413Mu03 Polyclonal Antibody to Mucin 1 (MUC1) WB; IHC; ICC; IP.
LAA413Mu71 Biotin-Linked Polyclonal Antibody to Mucin 1 (MUC1) WB; IHC; ICC.
SEA413Mu ELISA Kit for Mucin 1 (MUC1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA413Mu Multiplex Assay Kit for Mucin 1 (MUC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA413Mu01 ELISA Kit DIY Materials for Mucin 1 (MUC1) Main materials for "Do It (ELISA Kit) Yourself".