Multiplex Assay Kit for Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay)

CTF1

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-103 85
EDTA plasma(n=5) 78-93 89
heparin plasma(n=5) 80-91 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 88-96% 78-88% 91-105%
EDTA plasma(n=5) 95-105% 88-95% 84-105% 90-101%
heparin plasma(n=5) 79-93% 90-101% 79-98% 96-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CT1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
European Journal of Preventive Cardiology Exercise rescued chronic kidney disease by attenuating cardiac hypertrophy through the cardiotrophin-1 -> LIFR/gp 130 -> JAK/STAT3 pathway. PubMed: 23064267
Journal of Hypertension Is There Any Additional Cardiotrophin 1 Increase With Obesity in Hypertensive Patients?: Pp. 12.466 Lww: Source
Journal of Hypertension Cardiotrophyn 1 Levels in Nondipper Hypertensive Patients: Pp. 12.464 Lww: Source
American Journal of Hypertension Association Between Cardiotrophin 1 Levels and Central Blood Pressure in Untreated Patients With Essential Hypertension Pubmed: 24401751
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599
Food & Function Pterostilbene improves glycaemic control in rats fed an obesogenic diet: involvement of skeletal muscle and liver PubMed: 25998070
International journal of cardiology Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535
Soldan sağa şantlı doğumsal kalp hastalıklı çocuklarda şant miktarı ile kardiyotrofin–1 ilişkisi Doi: 10.5505/deutfd.2018.25338.30074
Annals of Nutrition and Metabolism Effect of Salt Intake on the Serum Cardiotrophin-1 Levels in Chinese Adults Pubmed: 30408810
Atherosclerotic diseases Cardiotrophin-1 and leptin as cardiovascular risk markers in male patients with obstructive sleep apnea syndrome Pubmed: 30775602
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