ELISA Kit for Cardiotrophin 1 (CT1)

CTF1

Specificity

This assay has high sensitivity and excellent specificity for detection of Cardiotrophin 1 (CT1).
No significant cross-reactivity or interference between Cardiotrophin 1 (CT1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cardiotrophin 1 (CT1) and the recovery rates were calculated by comparing the measured value to the expected amount of Cardiotrophin 1 (CT1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-92 88
EDTA plasma(n=5) 92-105 96
heparin plasma(n=5) 91-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cardiotrophin 1 (CT1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-101% 98-105% 90-97% 92-102%
EDTA plasma(n=5) 82-90% 83-97% 97-105% 86-98%
heparin plasma(n=5) 79-89% 98-105% 81-89% 78-88%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
European Journal of Preventive Cardiology Exercise rescued chronic kidney disease by attenuating cardiac hypertrophy through the cardiotrophin-1 -> LIFR/gp 130 -> JAK/STAT3 pathway. PubMed: 23064267
Journal of Hypertension Is There Any Additional Cardiotrophin 1 Increase With Obesity in Hypertensive Patients?: Pp. 12.466 Lww: Source
Journal of Hypertension Cardiotrophyn 1 Levels in Nondipper Hypertensive Patients: Pp. 12.464 Lww: Source
American Journal of Hypertension Association Between Cardiotrophin 1 Levels and Central Blood Pressure in Untreated Patients With Essential Hypertension Pubmed: 24401751
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599
Food & Function Pterostilbene improves glycaemic control in rats fed an obesogenic diet: involvement of skeletal muscle and liver PubMed: 25998070
International journal of cardiology Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535
Soldan sağa şantlı doğumsal kalp hastalıklı çocuklarda şant miktarı ile kardiyotrofin–1 ilişkisi Doi: 10.5505/deutfd.2018.25338.30074
Annals of Nutrition and Metabolism Effect of Salt Intake on the Serum Cardiotrophin-1 Levels in Chinese Adults Pubmed: 30408810
Atherosclerotic diseases Cardiotrophin-1 and leptin as cardiovascular risk markers in male patients with obstructive sleep apnea syndrome Pubmed: 30775602
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