Multiplex Assay Kit for Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 79-89 83
heparin plasma(n=5) 94-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-103% 84-102% 97-104% 99-105%
EDTA plasma(n=5) 93-101% 80-99% 81-99% 89-96%
heparin plasma(n=5) 86-93% 94-103% 80-90% 81-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:IL31) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
allergy and clinical immunology Increased serum levels of Interleukin-31 could modulate inflammatory processes in multiple sclerosis Siaic-Review:Source
BMC Immunol Increased levels of interleukin 31 (IL-31) in osteoporosis PubMed: 2644965
Sci Rep. Interleukin-31 expression and relation to disease severity in human asthma pmc:PMC4783779
Exp Dermatol. Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice. pubmed:28191683
Nat Commun.  The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. pubmed:28067314
Experimental Dermatology Immediate-type allergic and protease-media ted reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice doi:10.1111
アトピー性皮膚炎の痒みの発症機序に関する研究
Indian Journal of Public Health Research & Development Determination the Role of Interleukin 31 (IL-31) Levels in Three Real Allergic Diseases (Asthma, Rhinitis, Urticaria)
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
EPB179Ra61 Eukaryotic Interleukin 31 (IL31) Positive Control; Immunogen; SDS-PAGE; WB.
RPB179Ra01 Recombinant Interleukin 31 (IL31) Positive Control; Immunogen; SDS-PAGE; WB.
PAB179Ra01 Polyclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC; IP.
LAB179Ra81 FITC-Linked Polyclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC; IF.
LAB179Ra71 Biotin-Linked Polyclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC.
MAB179Ra21 Monoclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC; IP.
SEB179Ra ELISA Kit for Interleukin 31 (IL31) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB179Ra Multiplex Assay Kit for Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB179Ra01 ELISA Kit DIY Materials for Interleukin 31 (IL31) Main materials for "Do It (ELISA Kit) Yourself".