Multiplex Assay Kit for Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Metastin; KiSS-1 Metastasis-Suppressor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 92-101 97
heparin plasma(n=5) 94-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-88% 80-105% 85-101% 84-98%
EDTA plasma(n=5) 82-92% 97-105% 89-101% 82-101%
heparin plasma(n=5) 80-90% 80-94% 89-97% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:KISS1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Mol Endocrinol. LIM-homeodomain transcription factor Isl-1 mediates kisspeptin&#039;s effect on insulin secretion in mice. Pubmed:24956377
临床儿科杂志 双酚A 与女童性早熟的关系 Fileup: Pdf
J Pediatr Endocrinol Metab The relation of urinary bisphenol A with kisspeptin in girls diagnosed with central precocious puberty and premature thelarche PubMed: 26565542
Archives of Physiology and Biochemistry Circulating levels of leptin, nesfatin-1 and kisspeptin in postmenopausal obese women Pubmed:27011110
International Journal of Endocrinology The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162
Journal of Clinical Research in Pediatric Endocrinology Basal Serum Neurokinin B Levels in Differentiating Idiopathic Central Precocious Puberty from Premature Thelarche. pubmed:28008860
BioMed Research International Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China
OBESITY SURGERY Lack of Improvement of Sperm Characteristics in Obese Males After Obesity Surgery Despite the Beneficial Changes Observed in Reproductive Hormones Pubmed: 30806913
International Journal of Endocrinology Serum Kisspeptin and Its Relation to Metabolic Parameters and Glucose Metabolism in Prepubertal and Pubertal Obese Children Pubmed: 33224197
journal of reproduction and fertility KISS1R Signaling Modulates Gonadotropin Sensitivity in Mouse Leydig Cell Pubmed: 33112765
Beyond GnRH, LH and FSH: the role of kisspeptin on hypothalalmic-3 pituitary gonadal (HPG) axis pathology and diagnostic consideration 4
Medeni Med J Kisspeptin and Hematologic Parameters as Predictive Biomarkers for First-Trimester Abortions 34239761
Arch Gynecol Obstet Comparison of the Kisspeptin levels in early onset preeclampsia and late-onset preeclampsia Pubmed:35039882
J Pediatr Endocrinol Metab Serum kisspeptin, leptin, neuropeptide Y, and neurokinin B levels in adolescents with polycystic ovary syndrome Pubmed:35170267
Genes Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis Pubmed:35205340
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC559Hu01 Recombinant Kisspeptin 1 (KISS1) Positive Control; Immunogen; SDS-PAGE; WB.
CPC559Hu21 OVA Conjugated Kisspeptin 1 (KISS1) Immunogen; SDS-PAGE; WB.
PAC559Hu01 Polyclonal Antibody to Kisspeptin 1 (KISS1) WB; IHC; ICC; IP.
PAC559Hu08 Polyclonal Antibody to Kisspeptin 1 (KISS1) WB; IHC; ICC; IP.
MAC559Hu21 Monoclonal Antibody to Kisspeptin 1 (KISS1) WB; IHC; ICC; IP.
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SEC559Hu ELISA Kit for Kisspeptin 1 (KISS1) Enzyme-linked immunosorbent assay for Antigen Detection.
CEC559Hu ELISA Kit for Kisspeptin 1 (KISS1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC559Hu Multiplex Assay Kit for Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC559Hu01 ELISA Kit DIY Materials for Kisspeptin 1 (KISS1) Main materials for "Do It (ELISA Kit) Yourself".