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ELISA Kit DIY Materials for Kisspeptin 1 (KISS1)

Metastin; KiSS-1 Metastasis-Suppressor

  • Product No.KSC559Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity13.1pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Kisspeptin 1 (KISS1). No significant cross-reactivity or interference between Kisspeptin 1 (KISS1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Mol Endocrinol. LIM-homeodomain transcription factor Isl-1 mediates kisspeptin's effect on insulin secretion in mice. Pubmed:24956377
临床儿科杂志 双酚A 与女童性早熟的关系 Fileup: Pdf
J Pediatr Endocrinol Metab The relation of urinary bisphenol A with kisspeptin in girls diagnosed with central precocious puberty and premature thelarche PubMed: 26565542
Archives of Physiology and Biochemistry Circulating levels of leptin, nesfatin-1 and kisspeptin in postmenopausal obese women Pubmed:27011110
International Journal of Endocrinology The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162
Journal of Clinical Research in Pediatric Endocrinology Basal Serum Neurokinin B Levels in Differentiating Idiopathic Central Precocious Puberty from Premature Thelarche. pubmed:28008860
BioMed Research International Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China
OBESITY SURGERY Lack of Improvement of Sperm Characteristics in Obese Males After Obesity Surgery Despite the Beneficial Changes Observed in Reproductive Hormones Pubmed: 30806913
International Journal of Endocrinology Serum Kisspeptin and Its Relation to Metabolic Parameters and Glucose Metabolism in Prepubertal and Pubertal Obese Children Pubmed: 33224197
journal of reproduction and fertility KISS1R Signaling Modulates Gonadotropin Sensitivity in Mouse Leydig Cell Pubmed: 33112765
Beyond GnRH, LH and FSH: the role of kisspeptin on hypothalalmic-3 pituitary gonadal (HPG) axis pathology and diagnostic consideration 4
Medeni Med J Kisspeptin and Hematologic Parameters as Predictive Biomarkers for First-Trimester Abortions 34239761
Arch Gynecol Obstet Comparison of the Kisspeptin levels in early onset preeclampsia and late-onset preeclampsia Pubmed:35039882
J Pediatr Endocrinol Metab Serum kisspeptin, leptin, neuropeptide Y, and neurokinin B levels in adolescents with polycystic ovary syndrome Pubmed:35170267
Genes Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis Pubmed:35205340
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC559Hu01 Recombinant Kisspeptin 1 (KISS1) Positive Control; Immunogen; SDS-PAGE; WB.
CPC559Hu21 OVA Conjugated Kisspeptin 1 (KISS1) Immunogen; SDS-PAGE; WB.
PAC559Hu01 Polyclonal Antibody to Kisspeptin 1 (KISS1) WB; IHC; ICC; IP.
PAC559Hu08 Polyclonal Antibody to Kisspeptin 1 (KISS1) WB; IHC; ICC; IP.
MAC559Hu21 Monoclonal Antibody to Kisspeptin 1 (KISS1) WB; IHC; ICC; IP.
MAC559Hu22 Monoclonal Antibody to Kisspeptin 1 (KISS1) WB,IHC
SEC559Hu ELISA Kit for Kisspeptin 1 (KISS1) Enzyme-linked immunosorbent assay for Antigen Detection.
CEC559Hu ELISA Kit for Kisspeptin 1 (KISS1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC559Hu Multiplex Assay Kit for Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC559Hu01 ELISA Kit DIY Materials for Kisspeptin 1 (KISS1) Main materials for "Do It (ELISA Kit) Yourself".