Mini Samples ELISA Kit for Angiopoietin 1 (ANGPT1)
ANG1; AGP1; AGPT; ANG-I
- Product No.MEA008Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.24-15ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.09ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Mini Samples Angiopoietin 1 (ANGPT1).
No significant cross-reactivity or interference between Mini Samples Angiopoietin 1 (ANGPT1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Mini Samples Angiopoietin 1 (ANGPT1) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Angiopoietin 1 (ANGPT1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Angiopoietin 1 (ANGPT1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Angiopoietin 1 (ANGPT1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Angiopoietin 1 (ANGPT1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×4.5mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
|Theriogenology||Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article|
|J Atheroscler Thromb||High Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction PubMed: 25735431|
|Archives of Biological Sciences||Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu|
|Am J Transl Res||Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice Pubmed:27347310|
|Clinical research||Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19|
|Turkish Journal of Medicl Sciences||A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer pubmed:28425270|
|Sci Rep.||Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. pubmed:28276491|
|PeerJ||The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker pubmed:28584715|
|Molecular&cellular proteomics||Prediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry pubmed:28550167|
|Tumor Biology||Cervical cancer cell–derived angiopoietins promote tumor progression pubmed:28720059|
|Free Patents online||RHEUMATOID ARTHRITIS RELATED BIOMARKER 20170350884|
|Anesthesiology||Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277|
|British Journal of Anaesthesia||Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049|
|The Journal of Biological Chemistry||Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice Pubmed:29929978|
|Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse Doi: 10.4143/crt.2018.214|
|BIOLOGY OF REPRODUCTION||Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors Pubmed: 30929911|
|Scientific Reports||Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827|
|Biochem Biophys Res Commun||Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes Pubmed: 32800538|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA008Hu01||Recombinant Angiopoietin 1 (ANGPT1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA008Hu02||Recombinant Angiopoietin 1 (ANGPT1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA008Hu03||Recombinant Angiopoietin 1 (ANGPT1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA008Hu01||Polyclonal Antibody to Angiopoietin 1 (ANGPT1)||WB; IHC; ICC; IP.|
|PAA008Hu02||Polyclonal Antibody to Angiopoietin 1 (ANGPT1)||WB; IHC; ICC; IP.|
|LAA008Hu71||Biotin-Linked Polyclonal Antibody to Angiopoietin 1 (ANGPT1)||WB; IHC; ICC.|
|MAA008Hu21||Monoclonal Antibody to Angiopoietin 1 (ANGPT1)||WB; IHC; ICC; IP.|
|MAA008Hu22||Monoclonal Antibody to Angiopoietin 1 (ANGPT1)||WB; IHC; ICC; IP.|
|LAA008Hu72||Biotin-Linked Monoclonal Antibody to Angiopoietin 1 (ANGPT1)||WB; IHC; ICC.|
|SEA008Hu||ELISA Kit for Angiopoietin 1 (ANGPT1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEA008Hu||Mini Samples ELISA Kit for Angiopoietin 1 (ANGPT1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA008Hu||CLIA Kit for Angiopoietin 1 (ANGPT1)||Chemiluminescent immunoassay for Antigen Detection.|