Mini Samples ELISA Kit for Angiopoietin 1 (ANGPT1)

ANG1; AGP1; AGPT; ANG-I

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Angiopoietin 1 (ANGPT1).
No significant cross-reactivity or interference between Mini Samples Angiopoietin 1 (ANGPT1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Angiopoietin 1 (ANGPT1) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Angiopoietin 1 (ANGPT1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-104 87
EDTA plasma(n=5) 88-97 94
heparin plasma(n=5) 79-104 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Angiopoietin 1 (ANGPT1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Angiopoietin 1 (ANGPT1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Angiopoietin 1 (ANGPT1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-98% 93-103% 90-97% 78-104%
EDTA plasma(n=5) 91-103% 90-97% 94-103% 90-97%
heparin plasma(n=5) 97-105% 79-104% 89-96% 83-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Theriogenology Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article
J Atheroscler Thromb High Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction PubMed: 25735431
Archives of Biological Sciences Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu
Am J Transl Res Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice Pubmed:27347310
Clinical research Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19
Turkish Journal of Medicl Sciences A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer pubmed:28425270
Sci Rep.  Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. pubmed:28276491
PeerJ  The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker pubmed:28584715
Molecular&cellular proteomics Prediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry pubmed:28550167
Tumor Biology Cervical cancer cell–derived angiopoietins promote tumor progression pubmed:28720059
Free Patents online RHEUMATOID ARTHRITIS RELATED BIOMARKER 20170350884
Anesthesiology Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277
British Journal of Anaesthesia Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049
The Journal of Biological Chemistry Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice Pubmed:29929978
Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse Doi: 10.4143/crt.2018.214
BIOLOGY OF REPRODUCTION Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors Pubmed: 30929911
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827
Biochem Biophys Res Commun Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes Pubmed: 32800538
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