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Mini Samples ELISA Kit for Interferon Gamma (IFNg)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Interferon Gamma (IFNg).
No significant cross-reactivity or interference between Mini Samples Interferon Gamma (IFNg) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Interferon Gamma (IFNg) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interferon Gamma (IFNg) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 96
EDTA plasma(n=5) 90-98 94
heparin plasma(n=5) 91-105 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interferon Gamma (IFNg) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interferon Gamma (IFNg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 88-97% 88-104% 93-101%
EDTA plasma(n=5) 88-96% 83-99% 95-103% 87-101%
heparin plasma(n=5) 87-94% 79-101% 95-105% 80-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

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RPA049Hu01 Recombinant Interferon Gamma (IFNg) Positive Control; Immunogen; SDS-PAGE; WB.
PAA049Hu01 Polyclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC; IP.
PAA049Hu06 Polyclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC; IP.
FAA049Hu01 Anti-Interferon Gamma (IFNg) Polyclonal Antibody Flow cytometry.
LAA049Hu71 Biotin-Linked Polyclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC.
LAA049Hu81 FITC-Linked Polyclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC; IF.
MAA049Hu22 Monoclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC; IP.
RAA049Hu21 Recombinant Antibody to Interferon Gamma (IFNg) WB; IF; ICC; IHC; IP; FCM.
MAA049Hu21 Monoclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC; IP.
MAA049Hu26 Monoclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC; IP.
FAA049Hu02 Anti-Interferon Gamma (IFNg) Monoclonal Antibody FCM
LAA049Hu72 Biotin-Linked Monoclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC.
LAA049Hu82 FITC-Linked Monoclonal Antibody to Interferon Gamma (IFNg) WB; IHC; ICC; IF.
SEA049Hu ELISA Kit for Interferon Gamma (IFNg) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA049Hu High Sensitive ELISA Kit for Interferon Gamma (IFNg) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA049Hu Mini Samples ELISA Kit for Interferon Gamma (IFNg) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA049Hu ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA049Hu CLIA Kit for Interferon Gamma (IFNg) Chemiluminescent immunoassay for Antigen Detection.
LMA049Hu Multiplex Assay Kit for Interferon Gamma (IFNg) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA049Hu01 Antibody Pair for Interferon Gamma (IFNg) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA049Hu01 ELISA Kit DIY Materials for Interferon Gamma (IFNg) Main materials for "Do It (ELISA Kit) Yourself".