Mini Samples ELISA Kit for Galectin 3 (GAL3)

LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Galectin 3 (GAL3).
No significant cross-reactivity or interference between Mini Samples Galectin 3 (GAL3) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Galectin 3 (GAL3) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Galectin 3 (GAL3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-101 94
EDTA plasma(n=5) 90-99 96
heparin plasma(n=5) 84-102 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Galectin 3 (GAL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Galectin 3 (GAL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Galectin 3 (GAL3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 94-103% 80-101% 80-91%
EDTA plasma(n=5) 80-101% 93-103% 79-95% 92-99%
heparin plasma(n=5) 98-105% 83-97% 93-101% 94-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Clinica Chimica Acta Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599
Scientifi Reportcs Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices PubMed: 26582585
Clin Chim Acta Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population PubMed: 25578396
JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4. pubmed:28431936
昆明医科大学学报 半乳糖凝集素3 在COPD 和肺癌中的表达及其意义. 2095610X
Archives of Medical Science Can we define migraine patients with blood high-sensitivity C-reactive protein and galectin-3 levels in the emergency department? publication:305625095
Biosensors & Bioelectronics A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@AuNPs nanocomposites and a novel AuPt-methylene blue nanorod. pubmed:29096363
Scientific Reports The profile of selected single nucleotide polymorphisms in patients with hypertension and heart failure with preserved and mid-range ejection fraction. pubmed:28827564
Clinica Chimica Acta Galectin-3 in patients with coronary heart disease and atrial fibrillation Pubmed:29287900
Advances in Experimental Medicine and Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease Pubmed:29429028
FASEB Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922
american journal of physiology-heart and circulatory physiology Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus Pubmed: 32216615
Cardiology Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation Pubmed: 32516780
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