Mini Samples ELISA Kit for Galectin 3 (GAL3)
LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29
- Product No.MEA303Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Mini Samples Galectin 3 (GAL3).
No significant cross-reactivity or interference between Mini Samples Galectin 3 (GAL3) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Mini Samples Galectin 3 (GAL3) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Galectin 3 (GAL3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Galectin 3 (GAL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Galectin 3 (GAL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Galectin 3 (GAL3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×4.5mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
|Clinica Chimica Acta||Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395|
|Scientific Reports||Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153|
|Int J Mol Sci||The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599|
|Scientifi Reportcs||Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices PubMed: 26582585|
|Clin Chim Acta||Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population PubMed: 25578396|
|JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION||Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4. pubmed:28431936|
|昆明医科大学学报||半乳糖凝集素3 在COPD 和肺癌中的表达及其意义. 2095610X|
|Archives of Medical Science||Can we define migraine patients with blood high-sensitivity C-reactive protein and galectin-3 levels in the emergency department? publication:305625095|
|Biosensors & Bioelectronics||A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@AuNPs nanocomposites and a novel AuPt-methylene blue nanorod. pubmed:29096363|
|Scientific Reports||The profile of selected single nucleotide polymorphisms in patients with hypertension and heart failure with preserved and mid-range ejection fraction. pubmed:28827564|
|Clinica Chimica Acta||Galectin-3 in patients with coronary heart disease and atrial fibrillation Pubmed:29287900|
|Advances in Experimental Medicine and Biology||Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease Pubmed:29429028|
|FASEB Journal||Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922|
|american journal of physiology-heart and circulatory physiology||Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus Pubmed: 32216615|
|Cardiology||Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation Pubmed: 32516780|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA303Hu01||Recombinant Galectin 3 (GAL3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|EPA303Hu61||Eukaryotic Galectin 3 (GAL3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA303Hu01||Active Galectin 3 (GAL3)||Cell culture; Activity Assays.|
|PAA303Hu01||Polyclonal Antibody to Galectin 3 (GAL3)||WB; IHC; ICC; IP.|
|LAA303Hu71||Biotin-Linked Polyclonal Antibody to Galectin 3 (GAL3)||WB; IHC; ICC.|
|LAA303Hu81||FITC-Linked Polyclonal Antibody to Galectin 3 (GAL3)||WB; IHC; ICC; IF.|
|MAA303Hu22||Monoclonal Antibody to Galectin 3 (GAL3)||WB; IHC; ICC; IP.|
|SEA303Hu||ELISA Kit for Galectin 3 (GAL3)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEA303Hu||Mini Samples ELISA Kit for Galectin 3 (GAL3)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA303Hu01||ELISA Kit DIY Materials for Galectin 3 (GAL3)||Main materials for "Do It(ELISA Kit) Yourself"|