Mini Samples ELISA Kit for Caspase 9 (CASP9)

APAF3; ICE-LAP6; MCH6; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 9; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Caspase 9 (CASP9).
No significant cross-reactivity or interference between Mini Samples Caspase 9 (CASP9) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Caspase 9 (CASP9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Caspase 9 (CASP9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Reprod Sci. Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells. Pubmed: 23420823
Molecular Neurobiology Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations Pubmed:25502462
BMC Cancer Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. pubmed:27461108
Exp Lung Res Stretch-induced apoptosis in rat alveolar epithelial cells is mediated by the intrinsic mitochondrial pathway. pubmed:28394655
The American Journal of Chinese Medicine Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells pubmed:28231747
Frontiers in Pharmacology Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus. pubmed:28553224
Clinical And Experimental Pharmacology And Physiology  Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway Pubmed:29266336
Environmental Toxicology Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice Pubmed: 31087429
European journal of medicinal chemistry Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity Pubmed: 31216504
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337
Environ Sci Pollut Res Int Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of … Pubmed: 32915453
SAGE Journals Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic …
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