Mini Samples ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)
ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3
- Product No.MEB684Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3).
No significant cross-reactivity or interference between Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×4.5mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
|The Journal of Biological Chemistry||Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophages Jcb: 280370|
|The Journal of Biological Chemistry||Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous Macrophages PubMed: PMC3323061|
|Journal of Reproductive Immunology||What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037|
|The Journal of Immunology||Alveolar Epithelial Cell–Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation Pubmed:27183597|
|Scientific Reports||The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathway pubmed:28970500|
|Journal of Immunological Methods||Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs. pubmed:29154772|
|Turkish Journal Of Medical Sciences||The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility Pubmed:29914245|
|Novel trajectories of Bromocriptine antidiabetic action: Leptin-IL-6/JAK2/p-STAT3/SOCS3, p-IR/p-AKT/GLUT4, PPARγ/Adiponectin, Nrf2/PARP-1, and GLP-1 articles:10.3389|
|JCI Insight||Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeutics Pubmed: 31619584|
|European Journal of Pharmacology||Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways Pubmed: 32067934|
|FASEB JOURNAL||Resident alveolar macrophage‐derived vesicular SOCS3 dampens allergic airway inflammation Pubmed: 32030817|
|molecular immunology||SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection Pubmed: 32645550|
|Experimental and Therapeutic Medicine||Changes and clinical significance of serum inflammatory factors in the treatment of pregnancy hypertension syndrome with magnesium sulfate combined with … Pubmed: 32742411|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB684Hu01||Recombinant Suppressors Of Cytokine Signaling 3 (SOCS3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB684Hu01||Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3)||WB; IHC; ICC; IP.|
|MAB684Hu22||Monoclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3)||WB; IHC; ICC; IP.|
|MEB684Hu||Mini Samples ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEB684Hu||ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)||Enzyme-linked immunosorbent assay for Antigen Detection.|