Mini Samples ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)

ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3).
No significant cross-reactivity or interference between Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-97 93
EDTA plasma(n=5) 86-101 98
heparin plasma(n=5) 93-105 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 92-99% 91-99% 79-91%
EDTA plasma(n=5) 99-105% 83-103% 83-93% 89-98%
heparin plasma(n=5) 97-105% 82-95% 81-101% 79-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
The Journal of Biological Chemistry Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophages Jcb: 280370
The Journal of Biological Chemistry Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous Macrophages PubMed: PMC3323061
Journal of Reproductive Immunology What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037
The Journal of Immunology Alveolar Epithelial Cell–Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation Pubmed:27183597
Scientific Reports The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathway pubmed:28970500
Journal of Immunological Methods Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs. pubmed:29154772
Turkish Journal Of Medical Sciences The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility Pubmed:29914245
Novel trajectories of Bromocriptine antidiabetic action: Leptin-IL-6/JAK2/p-STAT3/SOCS3, p-IR/p-AKT/GLUT4, PPARγ/Adiponectin, Nrf2/PARP-1, and GLP-1 articles:10.3389
JCI Insight Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeutics Pubmed: 31619584
European Journal of Pharmacology Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways Pubmed: 32067934
FASEB JOURNAL Resident alveolar macrophage‐derived vesicular SOCS3 dampens allergic airway inflammation Pubmed: 32030817
molecular immunology SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection Pubmed: 32645550
Experimental and Therapeutic Medicine Changes and clinical significance of serum inflammatory factors in the treatment of pregnancy hypertension syndrome with magnesium sulfate combined with … Pubmed: 32742411
NEUROCHEMICAL RESEARCH Modulation of the Nitric Oxide/BH4 Pathway Protects Against Irradiation-Induced Neuronal Damage 33755856
BIOMEDICINE & PHARMACOTHERAPY Potential contribution of ELISA and LFI Assays to assessment of the oxidative stress condition based on 8-oxodG biomarker 33957564
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB684Ra01 Recombinant Suppressors Of Cytokine Signaling 3 (SOCS3) Positive Control; Immunogen; SDS-PAGE; WB.
PAB684Ra01 Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3) WB; IHC; ICC; IP.
LAB684Ra71 Biotin-Linked Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3) WB; IHC; ICC.
MAB684Ra21 Monoclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3) WB; IHC; ICC; IP.
SEB684Ra ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB684Ra Mini Samples ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB684Ra Multiplex Assay Kit for Suppressors Of Cytokine Signaling 3 (SOCS3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.