Mini Samples ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Troponin T Type 2, Cardiac (TNNT2).
No significant cross-reactivity or interference between Mini Samples Troponin T Type 2, Cardiac (TNNT2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Troponin T Type 2, Cardiac (TNNT2) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Troponin T Type 2, Cardiac (TNNT2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 98
EDTA plasma(n=5) 89-96 93
heparin plasma(n=5) 84-93 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Troponin T Type 2, Cardiac (TNNT2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Troponin T Type 2, Cardiac (TNNT2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Troponin T Type 2, Cardiac (TNNT2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 93-101% 82-98% 79-93%
EDTA plasma(n=5) 94-102% 94-101% 90-98% 94-103%
heparin plasma(n=5) 81-103% 92-105% 89-103% 83-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
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Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats Wiley: source
European Journal of Pharmacology Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats ScienceDirect: S0014299909009984
Journal of translational medicine The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury PubMed: PMC3694448
Oral Diseases Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction Pubmed: 22834943
An International Journal of Pharmaceutical Sciences In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium Pubmed: 23700796
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Cardiovasc Toxicol. Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs Pubmed:24420420
British Journal of Pharmacology Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise Pubmed:25039894
Chemico-Biological Interactions Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways Pubmed:26807765
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The Journal of Maternal-Fetal & Neonatal Medicine Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. pubmed:27937003
PLoS One. Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. pubmed:27907155
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830
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