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Mini Samples ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2)
AME; AME1; HSD11K; HSD2; SDR9C3; Short Chain Dehydrogenase/Reductase Family 9C,Member 3; NAD-dependent 11-beta-hydroxysteroid dehydrogenase
- Product No.MEF183Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.63ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 588
US$ 840
US$ 3780
US$ 7140
US$ 58800
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2).
No significant cross-reactivity or interference between Mini Samples 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Journal of Allergy and Clinical Immunology | Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress Pubmed:27016803 |
| Bioorganic Chemistry | A novel derivatives of thiazol-4 (5H)-one and their activity in the inhibition of 11β-hydroxysteroid dehydrogenase type 1 Pubmed:29738970 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| MEF183Mu | Mini Samples ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) | Enzyme-linked immunosorbent assay for Antigen Detection. |