Polyclonal Antibody to Nitrotyrosine (NT)

3-Nitro-L-Tyrosine; 3-Nitrotyrosine

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NT. It has been selected for its ability to recognize NT in immunohistochemical staining and western blotting.

USAGE

Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular Medicine Reports 3-Nitrotyrosine, a biomarker for cardiomyocyte apoptosis induced by diabetic cardiomyopathy in a rat model Pubmed: 23970331
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835
J Histochem Cytochem Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats PubMed: 26385569
canadian journal of physiology and pharmacology Protective effects of Telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuro-inflammation and amyloidogenesis: possible role of brain derived neurotrophic factor 10.1139:cjpp-2017-0042
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
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