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ELISA Kit DIY Materials for Nitrotyrosine (NT)

3-Nitro-L-Tyrosine; 3-Nitrotyrosine

  • Product No.KSB863Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range9.88-800ng/mL
  • Applicable Sensitivity3.95ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1454  US$ 2424  US$ 4363  US$ 8484  US$ 14544 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Nitrotyrosine (NT). No significant cross-reactivity or interference between Nitrotyrosine (NT) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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J Histochem Cytochem Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats PubMed: 26385569
canadian journal of physiology and pharmacology Protective effects of Telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuro-inflammation and amyloidogenesis: possible role of brain derived neurotrophic factor 10.1139:cjpp-2017-0042
Orphanet journal of rare diseases The cardiovascular phenotype of adult patients with phenylketonuria Pubmed: 31492166
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPB863Ge11 BSA Conjugated Nitrotyrosine (NT) Immunogen; SDS-PAGE; WB.
CPB863Ge21 OVA Conjugated Nitrotyrosine (NT) Immunogen; SDS-PAGE; WB.
PAB863Ge01 Polyclonal Antibody to Nitrotyrosine (NT) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEB863Ge ELISA Kit for Nitrotyrosine (NT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB863Ge Multiplex Assay Kit for Nitrotyrosine (NT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB863Ge11 ELISA Kit DIY Materials for Nitrotyrosine (NT) Main materials for "Do It (ELISA Kit) Yourself".