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Recombinant Octamer Binding Transcription Factor 4 (OCT4)

POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3

  • Product No.RPA424Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass29.2kDa
  • Accurate Molecular Mass29kDa(Analysis of differences refer to the manual)
  • Residues & TagsSer129~Asn352 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point9.4
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 174  US$ 436  US$ 872  US$ 2616  US$ 6540 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Analytical Methods A universal glucometer-based biosensor for portable and quantitative detection of transcription factors Rsc: Source
Oncology Letters Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma Doi: 10.3892/ol.2018.9381
Stem Cell Research Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin
博士论文 Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products
Stem Cell Research Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a … Pubmed: 31862609
J Biochem Mol Toxicol Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effect Pubmed:35187753
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA424Mu02 Recombinant Octamer Binding Transcription Factor 4 (OCT4) Positive Control; Immunogen; SDS-PAGE; WB.
RPA424Mu01 Recombinant Octamer Binding Transcription Factor 4 (OCT4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA424Mu02 Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) WB; IHC; ICC; IP.
PAA424Mu01 Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) WB; IHC; ICC; IP.
SEA424Mu ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA424Mu Multiplex Assay Kit for Octamer Binding Transcription Factor 4 (OCT4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.