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Recombinant Carbonic Anhydrase I (CA1)

CA-I; CAI; Car1; CAB; Carbonic anhydrase B; Carbonate dehydratase I

  • Product No.RPA875Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Predicted Molecular Mass30.0kDa
  • Accurate Molecular Mass28kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla2~Phe261 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 152  US$ 380  US$ 760  US$ 2280  US$ 5700 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS ONE Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance PubMed: PMC3754973
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients Pubmed:26680509
Am J Trop Med Hyg. Serum Carbonic Anhydrase 1 is a Biomarker for Diagnosis of Human Schistosoma mansoni Infection. pubmed:28500821
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA875Hu01 Active Carbonic Anhydrase I (CA1) Cell culture; Activity Assays.
RPA875Hu01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA875Hu01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
LAA875Hu81 FITC-Linked Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
LAA875Hu71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC.
MAA875Hu22 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
SEA875Hu ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA875Hu Multiplex Assay Kit for Carbonic Anhydrase I (CA1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.