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Recombinant High Temperature Requirement Factor A4 (HTRA4)

HtrA Serine Peptidase 4

  • Product No.RPN048Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass20.9kDa
  • Accurate Molecular Mass21kDa(Analysis of differences refer to the manual)
  • Residues & TagsGly202~Leu362 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point4.5
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 140  US$ 350  US$ 700  US$ 2100  US$ 5250 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Placenta Upregulation of HtrA4 in the placentas of patients with severe pre-eclampsia PubMed: 22964307
J Clin Endocrinol Metab Human HtrA4 Expression Is Restricted to the Placenta, Is Significantly Up-Regulated in Early-Onset Preeclampsia, and High Levels of HtrA4 Cause Endothelial Dysfunction - See more at: http://press.endocrine.org/doi/abs/10.1210/jc.2014-3969#sthash.XcUOSx2Z.dpuf PubMed: 25946029
Journal of Biomolecular Screening HtrA3 Isoform-Specific ELISAs for Early Detection of Preeclampsia. pubmed:27932697
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPN048Hu01 Recombinant High Temperature Requirement Factor A4 (HTRA4) Positive Control; Immunogen; SDS-PAGE; WB.
PAN048Hu01 Polyclonal Antibody to High Temperature Requirement Factor A4 (HTRA4) WB; IHC; ICC; IP.
MAN048Hu21 Monoclonal Antibody to High Temperature Requirement Factor A4 (HTRA4) WB; IHC; ICC; IP.
SEN048Hu ELISA Kit for High Temperature Requirement Factor A4 (HTRA4) Enzyme-linked immunosorbent assay for Antigen Detection.
SCN048Hu CLIA Kit for High Temperature Requirement Factor A4 (HTRA4) Chemiluminescent immunoassay for Antigen Detection.
LMN048Hu Multiplex Assay Kit for High Temperature Requirement Factor A4 (HTRA4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.